PAK1 (Ab-212) antibody detects endogenous levels of total PAK1protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human PAK1around the phosphorylation site of threonine 212 (P-V-TP-P-T).
PAK1
Reactivity: Human
WB, IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Alexander, Yang, Hinds: "Cellular senescence requires CDK5 repression of Rac1 activity." in: Molecular and cellular biology, Vol. 24, Issue 7, pp. 2808-19, (2004) (PubMed).
Thiel, Reeder, Pfaff, Coleman, Sells, Chernoff: "Cell cycle-regulated phosphorylation of p21-activated kinase 1." in: Current biology : CB, Vol. 12, Issue 14, pp. 1227-32, (2002) (PubMed).
Rashid, Banerjee, Nikolic: "Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology." in: The Journal of biological chemistry, Vol. 276, Issue 52, pp. 49043-52, (2001) (PubMed).