PKR (Ab-446) antibody detects endogenous levels of total PKR protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human PKR around the phosphorylation site of threonine 446 (K-R-TP-R-S).
EIF2AK2
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Guerra, López-Fernández, García, Zaballos, Esteban et al.: "Human gene profiling in response to the active protein kinase, interferon-induced serine/threonine protein kinase (PKR), in infected cells. Involvement of the transcription factor ATF-3 IN ..." in: The Journal of biological chemistry, Vol. 281, Issue 27, pp. 18734-45, (2006) (PubMed).
Ryman, Meier, Nangle, Ragsdale, Korneeva, Rhoads, MacDonald, Klimstra: "Sindbis virus translation is inhibited by a PKR/RNase L-independent effector induced by alpha/beta interferon priming of dendritic cells." in: Journal of virology, Vol. 79, Issue 3, pp. 1487-99, (2005) (PubMed).
Pataer, Vorburger, Barber, Chada, Mhashilkar, Zou-Yang, Stewart, Balachandran, Roth, Hunt, Swisher: "Adenoviral transfer of the melanoma differentiation-associated gene 7 (mda7) induces apoptosis of lung cancer cells via up-regulation of the double-stranded RNA-dependent protein kinase (PKR)." in: Cancer research, Vol. 62, Issue 8, pp. 2239-43, (2002) (PubMed).