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|Antigen||CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD) Antibodies|
|Reactivity||Dog (Canine), Human Alternatives|
|Conjugate||This CRADD antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CRADD AntibodyTarget Details CRADD Application Details Handling Images
|Specificity||Detects an ~24 kD protein, corresponding to the apparent molecular mass of RAIDD on SDS-PAGE immunoblots. This 24 kD band, as well as a 67 kD band, can be specifically inhibited by the relevant peptide.|
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Dog (100%) Marmoset, Hamster, Elephant, Panda (93%) Monkey, Mouse (87%).|
A 15 residue synthetic peptide (C)NSSPSDRQINQLAQR based on the human RAIDD (residues 112-126) (1) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Dog (100%), Marmoset, Hamster, Elephant, Panda (93%), Monkey, Mouse (87%).
Type of Immunogen: Synthetic peptide - KLH conjugated
Target Details CRADDProduct Details anti-CRADD Antibody Application Details Handling Images back to top
|Alternative Name||CRADD / RAIDD (CRADD Antibody Abstract)|
Name/Gene ID: CRADD
Synonyms: CRADD, Death adaptor molecule RAIDD, RAIDD, MRT34
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-CRADD Antibody Target Details CRADD Handling Images back to top
Approved: IHC-P (10 μg/mL), WB (1:500)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CRADD Antibody Target Details CRADD Application Details Images back to top
|Buffer||BBS, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)|
|Storage Comment||Store at -20°C.|
ImagesProduct Details anti-CRADD Antibody Target Details CRADD Application Details Handling back to top