anti-Dog (Canine) Polymerase (DNA Directed), mu antibody for Western Blotting

Recommended Polymerase (DNA Directed), mu Antibody (supplied by: Log in to see )

Antigen
Polymerase (DNA Directed), mu (POLM) Antibodies
  • polmu
  • zgc:56122
  • POLM
  • Pol Mu
  • Tdt-N
  • AI462166
  • B230309I03Rik
  • polymerase (DNA directed), mu
  • DNA polymerase mu
  • polm
  • POLM
  • CC1G_09351
  • VDBG_09335
  • Polm
Epitope
Middle Region
6
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
38
16
16
1
1
1
Host
Rabbit
38
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
19
13
13
7
6
2
2
2
Options
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Available images

Catalog No. ABIN2785958
$ 289.00
Plus shipping costs $45.00

General

Antigen Polymerase (DNA Directed), mu (POLM) Antibodies
Epitope Middle Region
(6), (2), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(38), (16), (16), (1), (1), (1)
Host Rabbit
(38)
Clonality
Polyclonal
Conjugate Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(19), (13), (13), (7), (6), (2), (2), (2)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN981956
Sequence LRRFSRKEKG LWLNSHGLFD PEQKTFFQAA SEEDIFRHLG LEYLPPEQRN
Predicted Reactivity Cow: 79%, Dog: 92%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 86%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against POLM. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human POLM

Target Details

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Antigen
Alternative Name POLM (POLM Antibody Abstract)
Background POLM seems to act as an Ig mutase which is responsible for immunoglobulin (Ig) gene hypermutation.
Alias Symbols: Tdt-N, Pol Mu
Protein Interaction Partner: CEP55, IKZF3, PNMA1, PCNA, UBE2C, UBE2H, UBE2E1, UBE2D3, UBE2D2, UBE2D1,
Protein Size: 494
Molecular Weight 55 kDa
Gene ID 27434
NCBI Accession NM_013284, NP_037416
UniProt Q9NP87
Pathways DNA Damage Repair, Production of Molecular Mediator of Immune Response

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 494 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Juárez, Ruiz, Nick McElhinny, Ramsden, Blanco: "A specific loop in human DNA polymerase mu allows switching between creative and DNA-instructed synthesis." in: Nucleic acids research, Vol. 34, Issue 16, pp. 4572-82, 2006

Images

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Supplier Images
Western Blotting (WB) image for anti-Polymerase (DNA Directed), mu (POLM) (Middle Region) antibody (ABIN2785958) WB Suggested Anti-POLM Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positiv...