anti-Goat Vimentin antibody for ELISA

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
928
406
356
220
137
131
120
82
80
52
51
43
41
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Mouse
569
321
30
17
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
26
23
18
16
13
13
13
13
13
13
13
13
12
11
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
739
344
328
304
254
251
249
143
86
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN4365380
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4365381 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see VM452
1 ABIN4365382 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see VM452
1 ABIN4365414 ELISA Alexa Fluor 488 Mouse IgG1 Log in to see VM452
1 ABIN4365418 ELISA Biotin Mouse IgG1 Log in to see VM452
1 ABIN4365437 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 Log in to see SPM576
1 ABIN4365446 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 Log in to see SPM576
1 ABIN4365433 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 Log in to see SPM576
1 ABIN4365445 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 Log in to see SPM576
1 ABIN4365419 ELISA DyLight 650 Mouse IgG1 Log in to see VM452
1 ABIN4365415 ELISA Alexa Fluor 647 Mouse IgG1 Log in to see VM452
1 ABIN4365413 ELISA Alexa Fluor 405 Mouse IgG1 Log in to see VM452
1 ABIN4365420 ELISA DyLight 680 Mouse IgG1 Log in to see VM452
1 ABIN4365421 ELISA DyLight 488 Mouse IgG1 Log in to see VM452
1 ABIN4365423 ELISA DyLight 755 Mouse IgG1 Log in to see VM452
1 ABIN4365426 ELISA DyLight 550 Mouse IgG1 Log in to see VM452
1 ABIN4365428 ELISA DyLight 405 Mouse IgG1 Log in to see VM452
1 ABIN4365424 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 Log in to see VM452
1 ABIN4365416 ELISA Alexa Fluor 700 Mouse IgG1 Log in to see VM452
1 ABIN4365427 ELISA DyLight 350 Mouse IgG1 Log in to see VM452
1 ABIN4365432 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 Log in to see SPM576

General

Antigen Vimentin (VIM) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
(928), (406), (356), (220), (137), (131), (120), (82), (80), (52), (51), (43), (41), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(569), (321), (30), (17), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (26), (23), (18), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (11), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(739), (344), (328), (304), (254), (251), (249), (143), (86), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
Specificity This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Protein G purified
Immunogen A recominant protein fragment specific to Vimentin was used as the immunogen for the VM452 antibody.
Clone VM452
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
UniProt P08670
Research Area Lineage Markers, Cell/Tissue Markers, Cytoskeleton, Neural Stem Cell marker
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL, Immunohistochemistry-Frozen 0.5-1.0 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.