anti-Zebrafish (Danio rerio) Vimentin antibody for Immunocytochemistry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Chicken, Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
1039
462
415
275
179
168
156
117
115
74
59
53
49
47
42
29
25
12
7
6
3
2
2
1
1
1
1
1
Host
Mouse
657
347
29
14
12
11
10
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is conjugated to HRP
35
32
30
28
27
18
17
16
14
14
14
14
14
14
14
9
9
8
8
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
824
431
398
371
336
273
266
159
87
63
40
26
15
13
8
7
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN1042494
$ 470.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
1 ABIN1805484 ICC IHC IHC (fro) WB HRP Mouse IgG1 Log in to see RV202 0
1 ABIN1805483 FACS ICC IHC IHC (fro) FITC Mouse IgG1 Log in to see RV202 0
1 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
1 ABIN4365315 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 0
1 ABIN5681848 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 550 Mouse IgG1 Log in to see RV202 0
1 ABIN5681849 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 650 Mouse IgG1 Log in to see RV202 0
1 ABIN5681852 FACS ICC IF IHC IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see RV202 0
1 ABIN5681839 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see RV202 0
1 ABIN5681841 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see RV202 0
1 ABIN5681842 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see RV202 0
1 ABIN5681844 ICC IF IHC IHC (fro) IHC (p) WB Biotin Mouse IgG1 Log in to see RV202 0
1 ABIN5681845 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 Log in to see RV202 0
1 ABIN5681846 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Log in to see RV202 0
1 ABIN5681840 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see RV202 0
1 ABIN5681847 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Log in to see RV202 0
1 ABIN5681850 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Log in to see RV202 0
1 ABIN5681851 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 Log in to see RV202 0
1 ABIN5947609 FACS ICC IF IHC IHC (fro) IHC (p) Janelia Fluor® 549 Mouse IgG1 Log in to see RV202 0
1 ABIN5947610 FACS ICC IF IHC IHC (fro) IHC (p) Janelia Fluor® 646 Mouse IgG1 Log in to see RV202 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
(1039), (462), (415), (275), (179), (168), (156), (117), (115), (74), (59), (53), (49), (47), (42), (29), (25), (12), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(657), (347), (29), (14), (12), (11), (10), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is conjugated to HRP
(35), (32), (30), (28), (27), (18), (17), (16), (14), (14), (14), (14), (14), (14), (14), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(824), (431), (398), (371), (336), (273), (266), (159), (87), (63), (40), (26), (15), (13), (8), (7), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN1042494) Images
Specificity RV202-HRP reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-HRP is suitable for immunoblotting, immunocytochemistry on cell cultures and cytospins, as well as immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:10.
Characteristics Cytoskeletal,Tumour marker
Purification Purified
Clone RV202
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN1042494) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN1042494) Images back to top
Application Notes RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
Comment

RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN1042494) Images back to top
Format Liquid
Buffer PBS containing 0,1% BSA, 0.09% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-Vimentin antibody (ABIN1042494)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987