anti-Rat (Rattus) SMAD3 antibody for Flow Cytometry

Recommended SMAD3 Antibody (supplied by: Log in to see )

Antigen
SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • SMAD family member 3a
  • SMAD family member 3 L homeolog
  • SMAD3
  • smad3a
  • smad3.L
  • Smad3
Epitope
pSer423, pSer425
65
57
49
46
34
23
19
19
19
18
18
18
12
12
11
11
11
8
8
7
7
7
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
549
294
275
54
45
39
36
29
23
19
18
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
489
58
15
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
22
21
19
11
11
10
8
8
7
7
7
5
5
5
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
413
236
231
119
107
67
52
34
23
18
9
7
7
2
2
1
1
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Catalog No. ABIN4354683
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN214557 ELISA FACS IF IHC IHC (p) WB Rabbit Ser425, pSer423 Log in to see Polyclonal 0
1 ABIN4354682 ELISA FACS IHC IHC (p) WB Rabbit pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN222350 FACS IF IP ELISA WB Rabbit IgG N-Term Log in to see 0
1 ABIN5663007 FACS APC Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663019 FACS PE Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663010 ELISA FACS IHC IHC (p) WB DyLight 405 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663009 ELISA FACS IHC IHC (p) WB DyLight 350 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663003 ELISA FACS IHC IHC (p) WB Alexa Fluor 405 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663004 ELISA FACS IHC IHC (p) WB Alexa Fluor 488 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663016 ELISA FACS IHC IHC (p) FITC Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663011 ELISA FACS IHC IHC (p) WB DyLight 488 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663006 ELISA FACS IHC IHC (p) WB Alexa Fluor 700 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663005 ELISA FACS IHC IHC (p) WB Alexa Fluor 647 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663014 ELISA FACS IHC IHC (p) WB DyLight 680 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663013 ELISA FACS IHC IHC (p) WB DyLight 650 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663015 ELISA FACS IHC IHC (p) WB DyLight 755 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663012 ELISA FACS IHC IHC (p) WB DyLight 550 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663018 FACS PerCP Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0

General

Antigen SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
Epitope pSer423, pSer425
(65), (57), (49), (46), (34), (23), (19), (19), (19), (18), (18), (18), (12), (12), (11), (11), (11), (8), (8), (7), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(549), (294), (275), (54), (45), (39), (36), (29), (23), (19), (18), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(489), (58), (15)
Clonality
Polyclonal
Conjugate This SMAD3 antibody is un-conjugated
(22), (21), (19), (11), (11), (10), (8), (8), (7), (7), (7), (5), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(413), (236), (231), (119), (107), (67), (52), (34), (23), (18), (9), (7), (7), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-SMAD3 Antibody

Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN4354683) Images
Specificity This antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues.
Purification Immunogen affinity purified
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.

Target Details SMAD3

Product Details anti-SMAD3 Antibody Application Details Handling References for anti-SMAD3 antibody (ABIN4354683) Images back to top
Antigen
Alternative Name Smad3 (SMAD3 Antibody Abstract)
Background Gene Symbol: SMAD3
Gene ID 4088
NCBI Accession NC_000015
Pathways Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy

Application Details

Product Details anti-SMAD3 Antibody Target Details SMAD3 Handling References for anti-SMAD3 antibody (ABIN4354683) Images back to top
Application Notes Western Blot 1:2000-1:20000, Flow Cytometry 1:1:10 - 1:1000, ELISA 1:15000 - 1:30000, Immunohistochemistry 1:500, Immunohistochemistry-Paraffin 1:10-1:500Expect a band approximately 48 kDa in size corresponding - phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. Less than 0.2 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/mL TGF-beta for 1 hour is suggested.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details References for anti-SMAD3 antibody (ABIN4354683) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-SMAD3 antibody (ABIN4354683)

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling Images back to top
Product cited in:

Rogers, Moreno, Smith, Husson, Bukanov, Ledbetter, Budman, Lu, Wang, Ibraghimov-Beskrovnaya, Natoli: "Differences in the timing and magnitude of Pkd1 gene deletion determine the severity of polycystic kidney disease in an orthologous mouse model of ADPKD." in: Physiological reports, Vol. 4, Issue 12, 2016

Yamashita, Fatyol, Jin, Wang, Liu, Zhang: "TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta." in: Molecular cell, Vol. 31, Issue 6, pp. 918-24, 2008

Images

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN4354683) back to top
Supplier Images
Western Blotting (WB) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody (ABIN4354683) Western Blot: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Shows detection of e...
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pSer423), (pSer425) antibody (ABIN4354683) Immunohistochemistry: Smad3 [p Ser423, p Ser425] Antibody [NBP1-77836] - Used at 2.5 ...