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|Antigen||Teratocarcinoma-Derived Growth Factor 1 (TDGF1) Antibodies|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||This antibody detects CRIPTO (TDGF1) at N-term.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human TDGF1.|
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|Alternative Name||TDGF1 / CRIPTO (TDGF1 Antibody Abstract)|
|Background||TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers. This protein could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. It is preferentially expressed in gastric and colorectal carcinomas compared to their normal counterparts.Synonyms: CRGF, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1|
|Molecular Weight||21168 Da|
|Gene ID||6997, 9606|
|Pathways||EGFR Signaling Pathway|
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ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
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Parisi, DAndrea, Lago, Adamson, Persico, Minchiotti: "Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells." in: The Journal of cell biology, Vol. 163, Issue 2, pp. 303-14, 2003
Gray, Harrison, Vale: "Cripto forms a complex with activin and type II activin receptors and can block activin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 9, pp. 5193-8, 2003
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