anti-Human Teratocarcinoma-Derived Growth Factor 1 antibody for Enzyme Immunoassay

Recommended Teratocarcinoma-Derived Growth Factor 1 Antibody (supplied by: Log in to see )

Antigen
Teratocarcinoma-Derived Growth Factor 1 (TDGF1) Antibodies
  • CR
  • CRGF
  • CRIPTO
  • CR1
  • cripto
  • cb85
  • tdgf1
  • wu:fe01d04
  • zgc:109829
  • Cripto-1
  • cr1
  • cr1a
  • frl-1
  • frl1
  • xcr1
  • TDGF1
  • tdgf3
  • cripto-3
  • teratocarcinoma-derived growth factor 1
  • teratocarcinoma-derived growth factor 1, member 3 L homeolog
  • teratocarcinoma-derived growth factor 1, member 1
  • TDGF1
  • Tdgf1
  • tdgf1
  • tdgf1.3.L
  • tdgf1.1
Epitope
N-Term
25
14
8
7
6
5
4
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
167
62
55
2
2
Host
Rabbit
121
45
13
11
Clonality
Polyclonal
Conjugate
Un-conjugated
15
15
14
8
6
6
5
5
5
5
5
4
4
4
4
4
4
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
78
77
59
52
16
15
7
4
2
2
1
1
1
1
Options
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Catalog No. ABIN357430
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN1109214 EIA WB Mouse IgG1 Log in to see 3A6 0
4 ABIN498045 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 1
1 ABIN5551813 EIA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN2733385 EIA Rabbit IgG Log in to see Polyclonal 0

General

Antigen Teratocarcinoma-Derived Growth Factor 1 (TDGF1) Antibodies
Epitope N-Term
(25), (14), (8), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(167), (62), (55), (2), (2)
Host Rabbit
(121), (45), (13), (11)
Clonality
Polyclonal
Conjugate Un-conjugated
(15), (15), (14), (8), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(78), (77), (59), (52), (16), (15), (7), (4), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product details

Target Details Application Details Handling References Images
Specificity This antibody detects CRIPTO (TDGF1) at N-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human TDGF1.
Isotype Ig Fraction

Target Details

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Antigen
Alternative Name TDGF1 / CRIPTO (TDGF1 Antibody Abstract)
Background TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers. This protein could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. It is preferentially expressed in gastric and colorectal carcinomas compared to their normal counterparts.Synonyms: CRGF, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1
Molecular Weight 21168 Da
Gene ID 6997, 9606
UniProt P13385
Pathways EGFR Signaling Pathway

Application Details

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Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References

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Background publications

Parisi, DAndrea, Lago, Adamson, Persico, Minchiotti: "Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells." in: The Journal of cell biology, Vol. 163, Issue 2, pp. 303-14, 2003

Gray, Harrison, Vale: "Cripto forms a complex with activin and type II activin receptors and can block activin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 9, pp. 5193-8, 2003

Images

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Supplier Images
 image for anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (N-Term) antibody (ABIN357430) anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (N-Term) antibody
 image for anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (N-Term) antibody (ABIN357430) anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (N-Term) antibody (Image 2)