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His Tag Antibodies

(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays. Here are some common applications of antibodies that detect His tags:
  • Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
  • Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.

222 results :

Reactivity: Human WB Host: Mouse Monoclonal 6AT18 unconjugated
Pubmed 38 references
Catalog No. ABIN387750
 
Rockland 600-401-382 WB, ELISA, IHC, DB Host: Rabbit Polyclonal unconjugated
Pubmed 14 references
Catalog No. ABIN100492
Rockland Original
WB, ELISA Host: Rabbit Polyclonal unconjugated
Pubmed 16 references
Catalog No. ABIN1573881
 
WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated
Pubmed 30 references
Catalog No. ABIN387699
 
Rockland 200-301-382 WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2-G8 unconjugated
Pubmed 4 references
Catalog No. ABIN964552
Rockland Original
Reactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated
Pubmed 1 reference
Catalog No. ABIN652278
 
WB, ELISA, IP, DB, ISt Host: Mouse Monoclonal HIS-H8 unconjugated
Pubmed 5 references
Catalog No. ABIN559683
 
Rockland 200-304-382 WB, ELISA, FACS, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 Cy3
Pubmed 3 references
Catalog No. ABIN6720721
Rockland Original
Rockland 200-345-382 WB, ELISA, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 DyLight 800
Pubmed 1 reference
Catalog No. ABIN6720719
Rockland Original
Rockland 200-343-382 WB, ELISA, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 DyLight 649
Pubmed 5 references
Catalog No. ABIN6720637
Rockland Original
Rockland 200-303-382 WB, ELISA, IHC Host: Mouse Monoclonal 33D10-D2-G8 HRP
Pubmed 1 reference
Catalog No. ABIN964554
Rockland Original
WB, ELISA Host: Mouse Monoclonal 3G5H8 unconjugated
Catalog No. ABIN7131650
 
WB, IP, IF, IC Host: Mouse Monoclonal unconjugated
Catalog No. ABIN7304940
 
Rockland 200-310-382 FACS, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 Cy5
Catalog No. ABIN6720722
Rockland Original
Rockland 200-306-382 WB, ELISA, IHC Host: Mouse Monoclonal 33D10-D2-G8 Biotin
Pubmed 1 reference
Catalog No. ABIN964555
Rockland Original
Rockland 200-301-b90 WB, ELISA, IHC Host: Mouse Monoclonal 33D10-D2-G8 unconjugated
Catalog No. ABIN1043732
Rockland Original
Reactivity: Human WB Host: Mouse Monoclonal 6E6 unconjugated
Catalog No. ABIN5004651
 
Reactivity: Tag, Mouse, Rat, Bacteria WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Pubmed 2 references
Catalog No. ABIN727295
 
Reactivity: Various Species ELISA, IP, ICC, IHC (fro), CLIA Host: Mouse Monoclonal D10 unconjugated
Catalog No. ABIN7426805
 
WB, ELISA, IP, IF, IHC Host: Mouse Monoclonal HIS-H8 unconjugated
Pubmed 1 reference
Catalog No. ABIN2443717
 
  • Type Primary
    • Primary
  • Application
    • Western Blotting (WB)
    • ELISA
    • Immunoprecipitation (IP)
    • Flow Cytometry (FACS)
    • Immunohistochemistry (IHC)
    • Immunofluorescence (IF)
    • Fluorescence Microscopy (FM)
    • Immunocytochemistry (ICC)
    • FLISA
    • Immunofluorescence (Cultured Cells) (IF (cc))
    • Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    • Dot Blot (DB)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Immunoelectrophoresis (IEP)
    • Immunochromatography (IC)
    • Enzyme Immunoassay (EIA)
    • High-throughput Screening (HTS)
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Cellular Assay (CA)
    • Chemiluminescence Immunoassay (CLIA)
    • Fluorescence Resonance Energy Transfer Microscopy (FRET)
    • Immunostaining (ISt)
    • Microarray (MA)
    • Immunolocalization (IL)
    • Immunomicroscopy (IM)
    • Light Microscopy (LM)
  • Reactivity
    • Human
    • Tag
    • Mouse
    • Rat
    • Chicken
    • Cow
    • Duck
    • Monkey
    • Pig
    • Saccharomyces cerevisiae
    • Various Species
    • Bacteria
    • Rabbit
  • Cited in publications
  • Images available
  • Preservative free only
  • Carrier free only
  • Host
    • Mouse
    • Rabbit
    • Goat
    • Chicken
    • Human
    • Rat
    • Hamster
    • Sheep
  • Clonality
    • Monoclonal
    • Polyclonal
    • Chimeric
  • Clone
    • AD1-1-10
    • 3D5
    • 3D53D5
    • 33D10-D2-G8
    • 2405-H6
    • 5E639
    • 6G2A9
    • 6E6
    • HIS-H8
    • RM146
    • 16-002
    • 3G5H8
    • GT359
    • 11C113
    • 11n83
    • 13G7
    • 14B22
    • 17H03
    • 19b10
    • 1A056
    • 1A5
    • 1B9
    • 2F4B5
    • 2HIS-2H5
    • 3H27
    • 3H28
    • 55F8
    • 6A8H8
    • 6AT18
    • AS01
    • AT1D6
    • AY63
    • C2
    • D10
    • D3
    • DM1
    • FY12
    • GT161
    • HIS-003
    • HIS-H8-EH158
    • N144-14
    • PT0481R
    • RB19960
    • RMH01
  • Binding Specificity
    • Fab Region
  • Conjugate
    • Un-conjugated
    • HRP
    • Biotin
    • FITC
    • APC
    • Alkaline Phosphatase (AP)
    • DyLight 488
    • PE
    • CF®488A
    • CF®594
    • CF®640R
    • CF®680
    • CF®770
    • Cy3
    • Cy5
    • DyLight 405
    • DyLight 549
    • DyLight 649
    • DyLight 680
    • DyLight 800
    • AbBy Fluor® 350
    • AbBy Fluor® 488
    • AbBy Fluor® 555
    • AbBy Fluor® 594
    • AbBy Fluor® 647
    • AbBy Fluor® 680
    • AbBy Fluor® 750
    • Acridinium ester
    • Alexa Fluor 488
    • Alexa Fluor 555
    • Alexa Fluor 594
    • Alexa Fluor 647
    • Alexa Fluor 680
    • Alexa Fluor 750
    • Atto 550
    • Atto 647N
    • CF®405S
    • CF®568
    • CF®647
    • CF®680R
    • CF®740
    • Cy5.5
    • Cy7
    • DyLight 550
    • DyLight 650
    • PerCP
    • Sepharose
    • SureLight® R-PE
    • iFluor 488
    • iFluor 555
    • iFluor 647
  • Isotype
    • IgG
    • IgG1
    • IgG1 kappa
    • IgG2b
    • IgG2a
    • IgG3
    • Ig Fraction
    • IgG2
    • kappa
    • IgG kappa
    • IgG2a kappa
    • IgG2b kappa
    • IgG2c kappa
    • IgG3 kappa
    • IgY
    • IgG2 kappa
    • IgG4
    • IgG4 kappa
  • Protein Type
    • Recombinant Antibody
  • Format
    • Liquid
    • Lyophilized
  • Fragment
    • single-domain Antibody (sdAb)
  • Grade
    • MALS verified
  • Supplier
    • antibodies-online
    • Rockland
    • Biotium
    • USBio
    • GenScript
    • Acris
    • Columbia Biosciences
    • Signalway
    • Agrisera
    • Biomatik
    • Kamiya Biomedicals
    • RevMAb Biosciences
    • ACROBiosystems
    • Biospes
    • Boster Bio
    • Cohesion Biosciences
    • Enogene Biotech
    • NSJ Bioreagents
    • QED Bioscience
    • Antibodies Incorporated
    • Epitope Biotech
    • ProteoGenix

Latest Publications for our His Tag Antibodies

Wang, Li, Yang, Yang, Gu, Du, Yang, Wen, Park, Huang, He: "Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos." in: Pest management science, Vol. 80, Issue 12, pp. 6473-6482, (2024) (PubMed).

Keskin, Chen, Tsai, Du, Santos, Syu: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).

Winans, Wucher, Nadell: "Multispecies biofilm architecture determines bacterial exposure to phages." in: PLoS biology, Vol. 20, Issue 12, pp. e3001913, (2022) (PubMed).

Srivastava, Verhagen, Sasmal, Wasik, Diaz, Yu, Bensing, Khan, Khedri, Secrest, Sullam, Varki, Chen, Parrish, Varki: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).

Khan, Sasmal, Khedri, Secrest, Verhagen, Srivastava, Varki, Chen, Yu, Beddoe, Paton, Paton, Varki: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).

Minnaert, van Schie, Grootaert, Himpe, Devos, Weyts, De Keersmaecker, Braeckmans, Van Aken, Delanghe, De Smedt, Callewaert, Remaut: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).

Kaufmann, Brangsch, Kader, Saatz, Mangarova, Zacharias, Kempf, Schwaar, Ponader, Adams, Möckel, Botnar, Taupitz, Mägdefessel, Traub, Hamm, Weller, Makowski: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Fan, Kishore, Jansen-Olliges, Wang, Stahl, Psathaki, Harre, Warnecke, Weder, Preller, Zeilinger: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).

Daguer, Gonse, Shchukin, Farrera-Soler, Barluenga, Winssinger: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).

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