anti-Human PIAS1 antibody for Western Blotting

Recommended PIAS1 Antibody (supplied by: Log in to see )

Antigen
Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
  • 2900068C24Rik
  • Ddxbp1
  • GBP
  • DDXBP1
  • GU/RH-II
  • ZMIZ3
  • ddxbp1
  • gbp
  • pias
  • pias1-A
  • xPAIS1
  • xPIAS1
  • zmiz3
  • protein inhibitor of activated STAT 1
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT, 1a
  • protein inhibitor of activated STAT 1 L homeolog
  • PIAS1
  • Pias1
  • pias1a
  • pias1.L
Epitope
AA 636-651, C-Term
25
25
20
10
5
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
97
38
16
5
4
3
3
3
3
2
2
2
1
1
Host
Rabbit
92
8
Clonality
Polyclonal
Conjugate
This PIAS1 antibody is un-conjugated
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
95
62
57
19
11
5
5
3
2
2
Options
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Catalog No. ABIN3044474
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.8156 ABIN3032369 ICC IHC (p) WB Rabbit IgG AA 636-651 Log in to see Polyclonal 0
16.39519 ABIN3186436 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal 0
15.3156 ABIN388054 IHC (p) WB Rabbit Ig Fraction AA 100-130, N-Term Log in to see Polyclonal 7
13.8156 ABIN6264195 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
13.8156 ABIN1848832 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
12.579589 ABIN2148996 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.579589 ABIN6145592 WB Rabbit Log in to see Polyclonal 0
12.579589 ABIN6145593 IF WB Rabbit Log in to see Polyclonal 0
10.8156 ABIN2780998 WB Rabbit Middle Region Log in to see Polyclonal 1
10.8156 ABIN388055 IHC (p) WB Rabbit Ig Fraction AA 607-637, C-Term Log in to see Polyclonal 5
10.8156 ABIN615743 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
10.8156 ABIN2564529 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10.8156 ABIN2996740 IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN604736 ELISA IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
10 ABIN522007 PLA ELISA WB Mouse IgG2b kappa AA 543-651, partial Log in to see 4A4 0
7.8156 ABIN1533432 IHC ELISA WB Rabbit IgG AA 10-59 Log in to see Polyclonal 0
7.8156 ABIN1860208 ICC IHC WB Rabbit IgG AA 409-645 Log in to see Polyclonal 0
7.8156 ABIN2957720 IHC WB Rabbit N-Term Log in to see Polyclonal 0
7.8156 ABIN5585647 IHC (p) WB Rabbit Log in to see Polyclonal 0
7.8156 ABIN5585648 ELISA IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Protein Inhibitor of Activated STAT, 1 (PIAS1) Antibodies
Epitope AA 636-651, C-Term
(25), (25), (20), (10), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(97), (38), (16), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(92), (8)
Clonality
Polyclonal
Conjugate This PIAS1 antibody is un-conjugated
(4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(95), (62), (57), (19), (11), (5), (5), (3), (2), (2)
Pubmed 1 reference available
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Product Details anti-PIAS1 Antibody

Target Details PIAS1 Application Details Handling References for anti-PIAS1 antibody (ABIN3044474) Images
Purpose Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Sequence DTASIFGIIP DIISLD
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for E3 SUMO-protein ligase PIAS1(PIAS1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: protein inhibitor of activated STAT, 1
Protein Name: E3 SUMO-protein ligase PIAS1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human PIAS1(636-651aa DTASIFGIIPDIISLD), identical to the related mouse and rat sequences.
Isotype IgG
Plasmids, Primers & others

Target Details PIAS1

Product Details anti-PIAS1 Antibody Application Details Handling References for anti-PIAS1 antibody (ABIN3044474) Images back to top
Antigen
Alternative Name PIAS1 (PIAS1 Antibody Abstract)
Background E3 SUMO-protein ligase PIAS1 is an enzyme that in humans is encoded by the PIAS1 gene. It is mapped to 15q23. This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1(signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. PIAS1 inhibited STAT1-mediated gene activation in response to interferon when expressed in mammalian cells. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. On the other hand, PIAS1 also can function as a SUMO ligase, or possibly as a tightly bound regulator of it, toward p53.

Synonyms: DDXBP1 antibody|DEAD/H box binding protein 1 antibody|DEAD/H box-binding protein 1 antibody|E3 SUMO-protein ligase PIAS1 antibody|GBP antibody|Gu binding protein antibody|Gu-binding protein antibody|Pias1 antibody|PIAS1_HUMAN antibody|Protein inhibitor of activated STAT protein 1 antibody|RNA helicase II binding protein antibody|RNA helicase II-binding protein antibody
UniProt O75925
Pathways JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C

Application Details

Product Details anti-PIAS1 Antibody Target Details PIAS1 Handling References for anti-PIAS1 antibody (ABIN3044474) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details References for anti-PIAS1 antibody (ABIN3044474) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-PIAS1 antibody (ABIN3044474)

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling Images back to top
Product cited in:

Scortegagna, Berthon, Settas, Giannakou, Garcia, Li, James, Liddington, Vilches-Moure, Stratakis, Ronai: "The E3 ubiquitin ligase Siah1 regulates adrenal gland organization and aldosterone secretion." in: JCI insight, Vol. 2, Issue 23, 2017

Images

Product Details anti-PIAS1 Antibody Target Details PIAS1 Application Details Handling References for anti-PIAS1 antibody (ABIN3044474) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody (ABIN3044474) Anti-PIAS1 antibody, IHC(P) IHC(P): Rat Testis Tissue
Immunohistochemistry (IHC) image for anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody (ABIN3044474) Anti-PIAS1 antibody, ICC ICC: A549 Cell
Immunohistochemistry (IHC) image for anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody (ABIN3044474) Anti-PIAS1 antibody, IHC(P) IHC(P): Human Placenta Tissue
Western Blotting (WB) image for anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody (ABIN3044474) anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody (ABIN3044474) Anti-PIAS1 antibody, IHC(P) IHC(P): Rat Brain Tissue
Immunohistochemistry (IHC) image for anti-Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 636-651), (C-Term) antibody (ABIN3044474) Anti-PIAS1 antibody, IHC(P) IHC(P): Rat Brain Tissue