anti-Cow (Bovine) ATP2A2 antibody for ELISA

Recommended ATP2A2 Antibody (supplied by: Log in to see )

Antigen
ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Antibodies
  • atp2b
  • ca-p60a
  • dar
  • serca2
  • ATP2A2
  • ATP2B
  • DAR
  • DD
  • SERCA2
  • SERCA2A
  • ATP2
  • Serca2
  • SercaII
  • 9530097L16Rik
  • D5Wsu150e
  • SERCA2B
  • mKIAA4195
  • atp2a2
  • zgc:55380
  • ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 S homeolog
  • ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
  • ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
  • ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a
  • atp2a1.S
  • ATP2A2
  • Atp2a2
  • atp2a2a
Reactivity
Amphibian, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine), Virus
68
41
39
11
7
6
6
5
4
4
3
2
2
2
2
2
1
1
1
1
Host
Mouse
65
6
2
Clonality
Monoclonal
Conjugate
This ATP2A2 antibody is un-conjugated
3
3
3
2
2
2
Application
Blocking Peptide (BP), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Western Blotting (WB)
70
34
22
11
7
6
3
2
1
1
1
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Catalog No. ABIN152688
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General

Antigen ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) Antibodies
Reactivity Amphibian, Cow (Bovine), Dog (Canine), Guinea Pig, Hamster, Human, Mouse (Murine), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine), Virus
(68), (41), (39), (11), (7), (6), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(65), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ATP2A2 antibody is un-conjugated
(3), (3), (3), (2), (2), (2)
Application Blocking Peptide (BP), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Western Blotting (WB)
(70), (34), (22), (11), (7), (6), (3), (2), (1), (1), (1)
Pubmed 16 references available
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Product Details anti-ATP2A2 Antibody

Target Details ATP2A2 Application Details Handling References for anti-ATP2A2 antibody (ABIN152688) Images
Specificity Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This does not discriminate between the two isoforms.
Purification Immunogen affinity purified
Immunogen Purified canine cardiac sarcoplasmic reticulum.
Clone IID8
Isotype IgG1
Plasmids, Primers & others

Target Details ATP2A2

Product Details anti-ATP2A2 Antibody Application Details Handling References for anti-ATP2A2 antibody (ABIN152688) Images back to top
Antigen
Alternative Name ATP2A2 / SERCA2 (ATP2A2 Antibody Abstract)
Target Type Virus
Background Gene Symbol: ATP2A2
Gene ID 488
UniProt O46674
Pathways Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process

Application Details

Product Details anti-ATP2A2 Antibody Target Details ATP2A2 Handling References for anti-ATP2A2 antibody (ABIN152688) Images back to top
Application Notes Western Blot 1:500 - 1:5000, Flow Cytometry 1 μg/ 10^6 cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:200, Immunohistochemistry-Paraffin 1 μg/mL, Immunohistochemistry-Frozen 1:500, Blocking/Neutralizing 50 - 200 Molar excessWB: Detects an approx. 110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Not recommended for WB detection of rat SERCA2. IF: Staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization. May be useful in Flow.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-ATP2A2 Antibody Target Details ATP2A2 Application Details References for anti-ATP2A2 antibody (ABIN152688) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-ATP2A2 antibody (ABIN152688)

Product Details anti-ATP2A2 Antibody Target Details ATP2A2 Application Details Handling Images back to top
Product cited in:

Leatherbury, Yu, Chatterjee, Walker, Yu, Tian, Lo: "A novel mouse model of X-linked cardiac hypertrophy." in: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 6, pp. H2701-11, 2008

Chauvet, Tian, Husson, Grimm, Wang, Hiesberger, Hieseberger, Igarashi, Bennett, Ibraghimov-Beskrovnaya, Somlo, Caplan: "Mechanical stimuli induce cleavage and nuclear translocation of the polycystin-1 C terminus." in: The Journal of clinical investigation, Vol. 114, Issue 10, pp. 1433-43, 2004

Kjellgren, Ryan, Ohlendieck, Thornell, Pedrosa-Domellöf: "Sarco(endo)plasmic reticulum Ca2+ ATPases (SERCA1 and -2) in human extraocular muscles." in: Investigative ophthalmology & visual science, Vol. 44, Issue 12, pp. 5057-62, 2003

Adachi, Matsui, Xu, Kirber, Lazar, Sharov, Schöneich, Cohen: "Antioxidant improves smooth muscle sarco/endoplasmic reticulum Ca(2+)-ATPase function and lowers tyrosine nitration in hypercholesterolemia and improves nitric oxide-induced relaxation." in: Circulation research, Vol. 90, Issue 10, pp. 1114-21, 2002

Molnar, Seidler, Jona, Martonosi: "The binding of monoclonal and polyclonal antibodies to the Ca2(+)-ATPase of sarcoplasmic reticulum: effects on interactions between ATPase molecules." in: Biochimica et biophysica acta, Vol. 1023, Issue 2, pp. 147-67, 1990

Background publications

Poch, Leach, Snape, Cacic, MacLennan, Lytton: "Functional characterization of alternatively spliced human SERCA3 transcripts." in: The American journal of physiology, Vol. 275, Issue 6 Pt 1, pp. C1449-58, 1999

Hu, Yin, Zhang, Wright, Nixon, Wechsler, Spratt, Briggs: "Transcriptional regulation of phospholamban gene and translational regulation of SERCA2 gene produces coordinate expression of these two sarcoplasmic reticulum proteins during skeletal muscle phenotype switching." in: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11619-22, 1995

Sharp, McPherson, Dawson, Aoki, Campbell, Snyder: "Differential immunohistochemical localization of inositol 1,4,5-trisphosphate- and ryanodine-sensitive Ca2+ release channels in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 7, pp. 3051-63, 1993

Molnar, Varga, Jona, Seidler, Martonosi: "Immunological relatedness of the sarcoplasmic reticulum Ca(2+)-ATPase and the Na+,K(+)-ATPase." in: Biochimica et biophysica acta, Vol. 1103, Issue 2, pp. 281-95, 1992

Ohlendieck, Briggs, Lee, Wechsler, Campbell: "Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle." in: European journal of biochemistry / FEBS, Vol. 202, Issue 3, pp. 739-47, 1992

Plessers, Eggermont, Wuytack, Casteels: "A study of the organellar Ca2(+)-transport ATPase isozymes in pig cerebellar Purkinje neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 11, Issue 3, pp. 650-6, 1991

Spencer, Yu, Khan, Grover: "Expression of isoforms of internal Ca2+ pump in cardiac, smooth muscle and non-muscle tissues." in: Biochimica et biophysica acta, Vol. 1063, Issue 1, pp. 15-20, 1991

Ohlendieck, Ervasti, Snook, Campbell: "Dystrophin-glycoprotein complex is highly enriched in isolated skeletal muscle sarcolemma." in: The Journal of cell biology, Vol. 112, Issue 1, pp. 135-48, 1991

Movsesian, Leveille, Krall, Colyer, Wang, Campbell: "Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles." in: Journal of molecular and cellular cardiology, Vol. 22, Issue 12, pp. 1477-85, 1991

Briggs, Lee, Feher, Wechsler, Ohlendieck, Campbell: "Ca-ATPase isozyme expression in sarcoplasmic reticulum is altered by chronic stimulation of skeletal muscle." in: FEBS letters, Vol. 259, Issue 2, pp. 269-72, 1990

Jorgensen, Arnold, Pepper, Kahl, Mandel, Campbell: "A monoclonal antibody to the Ca2+-ATPase of cardiac sarcoplasmic reticulum cross-reacts with slow type I but not with fast type II canine skeletal muscle fibers: an immunocytochemical and immunochemical study." in: Cell motility and the cytoskeleton, Vol. 9, Issue 2, pp. 164-74, 1988

Images

Product Details anti-ATP2A2 Antibody Target Details ATP2A2 Application Details Handling References for anti-ATP2A2 antibody (ABIN152688) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [ABIN152688] - Both norm...
Immunofluorescence (IF) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [ABIN152688] - ...
Immunofluorescence (IF) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [ABIN152688] - ...
Immunofluorescence (IF) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (IID8) [ABIN152688] - ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [ABIN152688] - Both norm...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (IID8) [ABIN152688] - Both norm...
Western Blotting (WB) image for anti-ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) antibody (ABIN152688) Western Blot: SERCA2 ATPase Antibody (IID8) [ABIN152688] - Analysis of 25 ug of rat s...