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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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4 | ABIN2662981 | ELISA (Capture) Neut | PE | Hamster | IgG | Log in to see | TR75-89 | 7 | ||
1 | ABIN2662982 | ELISA (Capture) Neut | PE | Hamster | IgG | Log in to see | TR75-89 | 7 |
General |
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Antigen | Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B) Antibodies |
Epitope | Extracellular Domain Alternatives |
Reactivity | Mouse (Murine) Alternatives |
Host | Hamster Alternatives |
Clonality (Clone) | |
Conjugate | This TNFRSF1B antibody is un-conjugated Alternatives |
Application |
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Western Blotting (WB)
Alternatives
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4 references available |
Supplier | Log in to see |
Product Details anti-TNFRSF1B AntibodyTarget Details TNFRSF1B Application Details Handling References for anti-TNFRSF1B antibody (ABIN4900547) Images |
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Specificity | Detects mouse TNF RII/TNFRSF1B in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) TNF RII, recombinant mouse (rm) TNF RI, rhTNF RI, rm4-1BB, or rmOPG is observed. In Western blots, approximately 25 % cross-reactivity with rhDR3 and rhTNF RII is observed and no cross-reactivity with rm4-1BB, rmCD27, rmCD30, rmCD40, rmFas, rmGITR, rhHVEM, rhNGF R, rmOPG, rmRANK, rmTNF RI, rmTROY, or rmEDAR is observed. |
Purification | Protein A or G purified from hybridoma culture supernatant |
Sterility | 0.2 μm filtered |
Immunogen | E. coli-derived recombinant mouse TNF RII/TNFRSF1B, Extracellular domain |
Clone | TR7554 |
Isotype | IgG |
Plasmids, Primers & others | |
Target Details TNFRSF1BProduct Details anti-TNFRSF1B Antibody Application Details Handling References for anti-TNFRSF1B antibody (ABIN4900547) Images back to top |
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Antigen | |
Alternative Name | TNF RII/TNFRSF1B (TNFRSF1B Antibody Abstract) |
Background | Alternative Names: CD120b, CD120b antigen, Etanercept, p75 TNF receptor, p75TBPII, p75TNFR, soluble TNFR1B variant 1, TNFBRp80 TNF-alpha receptor, TNF-R2, TNFR2TNFR1B, TNF-R75, TNFR80, TNFRII, TNF-RII, TNF-R-II, TNFR-II, TNFRSF1B, tumor necrosis factor beta receptor, tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, tumor necrosis factor receptor superfamily member 1B, tumor necrosis factor receptor superfamily, member 1B, Tumor necrosis factor receptor type II |
Gene ID | 7133 |
Pathways | NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C |
Application DetailsProduct Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Handling References for anti-TNFRSF1B antibody (ABIN4900547) Images back to top |
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Application Notes |
Western Blot 1 μg/mL Flow Cytometry 1 μg/10^6 cells CyTOF-ready ELISA Capture (Matched Antibody Pair) 2-8 μg/mL |
Restrictions | For Research Use only |
HandlingProduct Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details References for anti-TNFRSF1B antibody (ABIN4900547) Images back to top |
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Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Preservative | Without preservative |
Storage | 4 °C,-20 °C,-80 °C |
Storage Comment | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution. |
Expiry Date | 12 months |
References for anti-TNFRSF1B antibody (ABIN4900547)Product Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details Handling Images back to top |
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Product cited in: |
Joedicke, Myers, Carmody, Messer, Wajant, Lang, Lang, Mak, Hasenkrug, Dittmer: "Activated CD8+ T cells induce expansion of Vβ5+ regulatory T cells via TNFR2 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 6, pp. 2952-60, 2014 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Mouse (Murine)). Osuchowski, Welch, Siddiqui, Remick: "Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 3, pp. 1967-74, 2006 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)). Osuchowski, Siddiqui, Copeland, Remick: "Sequential ELISA to profile multiple cytokines from small volumes." in: Journal of immunological methods, Vol. 302, Issue 1-2, pp. 172-81, 2005 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)). Ellaban, Bolgos, Remick: "Selective macrophage suppression during sepsis." in: Cellular immunology, Vol. 231, Issue 1-2, pp. 103-11, 2005 Method employed by authors: ELISA (ELISA), ELISA (ELISA), ELISA (ELISA) (Sample species: Mouse (Murine)). |
ImagesProduct Details anti-TNFRSF1B Antibody Target Details TNFRSF1B Application Details Handling References for anti-TNFRSF1B antibody (ABIN4900547) back to top |
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