anti-Human Manic Fringe antibody for Western Blotting

Recommended Manic Fringe Antibody (supplied by: Log in to see )

Antigen
MFNG Antibodies
  • AW546563
  • MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
  • MFNG
  • Mfng
Epitope
Middle Region
6
5
4
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
58
32
32
5
5
5
4
4
3
2
2
2
1
1
Host
Rabbit
49
10
2
1
Clonality
Polyclonal
Conjugate
This Manic Fringe antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
47
20
13
9
7
4
4
3
1
1
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Catalog No. ABIN2783425
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.079593 ABIN2783426 WB Rabbit C-Term Log in to see Polyclonal 1
3.079593 ABIN2782201 WB Rabbit Middle Region Log in to see Polyclonal 0
3.079593 ABIN3222780 WB Rabbit Log in to see Polyclonal 0
3.079593 ABIN1859809 ICC IHC WB Rabbit IgG AA 80-316 Log in to see Polyclonal 0
3.079593 ABIN395334 ELISA WB Mouse IgG2a kappa AA 214-292 Log in to see 2B11 0
3.079593 ABIN517894 WB Mouse AA 1-321, full length Log in to see Polyclonal 0
3.079593 ABIN633860 WB Rabbit Middle Region Log in to see Polyclonal 0
3.079593 ABIN633865 WB Rabbit C-Term Log in to see Polyclonal 0
3.079593 ABIN2463195 ELISA WB Rabbit Log in to see Polyclonal 0
3.079593 ABIN2463196 ELISA WB Rabbit Log in to see Polyclonal 0
3.079593 ABIN4893723 WB Rabbit C-Term Log in to see Polyclonal 0
3.079593 ABIN4893722 WB Rabbit Log in to see Polyclonal 0
3.079593 ABIN517895 WB Mouse AA 1-321, full length Log in to see Polyclonal 0
3.079593 ABIN561813 ELISA WB Mouse AA 214-291, partial Log in to see Polyclonal 0
3.079593 ABIN2909665 IF/ICC IHC IP WB Rabbit AA 80-316 Log in to see Polyclonal 0
3.079593 ABIN1850904 ELISA WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN799234 WB Rabbit AA 241-290 Log in to see Polyclonal 0
1 ABIN1913322 IHC IHC (p) WB Rabbit IgG AA 211-260 Log in to see Polyclonal 0
1 ABIN1913324 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN3185449 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0

General

Antigen MFNG Antibodies
Epitope Middle Region
(6), (5), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(58), (32), (32), (5), (5), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(49), (10), (2), (1)
Clonality
Polyclonal
Conjugate This Manic Fringe antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(47), (20), (13), (9), (7), (4), (4), (3), (1), (1)
Pubmed 1 reference available
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Product Details anti-Manic Fringe Antibody

Target Details Manic Fringe Application Details Handling References for anti-Manic Fringe antibody (ABIN2783425) Images
Blocking Peptide Blocking peptide for this product available: ABIN980025
Sequence MAPWASGSRF MDTSALIRLP DDCTMGYIIE CKLGGRLQPS PLFHSHLETL
Predicted Reactivity Cow: 79%, Dog: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against MFNG. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human MFNG
Plasmids, Primers & others

Target Details Manic Fringe

Product Details anti-Manic Fringe Antibody Application Details Handling References for anti-Manic Fringe antibody (ABIN2783425) Images back to top
Antigen
Alternative Name MFNG (MFNG Antibody Abstract)
Background MFNG is one of the evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene is a member of the fringe gene family which also includes Radical and Lunatic fringe. They all encode evolutionarily conserved secreted proteins that act in the Notch receptor pathway to demarcate boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta1,3 N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: -
Protein Interaction Partner: DLL1, JAG1, NOTCH2, NOTCH1,
Protein Size: 321
Molecular Weight 36 kDa
Gene ID 4242
NCBI Accession NM_002405, NP_002396
UniProt O00587
Pathways Notch Signaling

Application Details

Product Details anti-Manic Fringe Antibody Target Details Manic Fringe Handling References for anti-Manic Fringe antibody (ABIN2783425) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 321 AA

Restrictions For Research Use only

Handling

Product Details anti-Manic Fringe Antibody Target Details Manic Fringe Application Details References for anti-Manic Fringe antibody (ABIN2783425) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Manic Fringe antibody (ABIN2783425)

Product Details anti-Manic Fringe Antibody Target Details Manic Fringe Application Details Handling Images back to top
Background publications

Veeraraghavalu, Pett, Kumar, Nair, Rangarajan, Stanley, Krishna: "Papillomavirus-mediated neoplastic progression is associated with reciprocal changes in JAGGED1 and manic fringe expression linked to notch activation." in: Journal of virology, Vol. 78, Issue 16, pp. 8687-700, 2004

Images

Product Details anti-Manic Fringe Antibody Target Details Manic Fringe Application Details Handling References for anti-Manic Fringe antibody (ABIN2783425) back to top
Supplier Images
Western Blotting (WB) image for anti-MFNG (MFNG) (Middle Region) antibody (ABIN2783425) WB Suggested Anti-MFNG Antibody Titration: 0.2-1 ug/ml Positive Control: HepG2 cel...