anti-Rat (Rattus) BBC3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Epitope
AA 2-16
89
62
18
16
14
12
10
9
8
8
5
4
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
287
121
107
18
1
1
1
Host
Rabbit
254
24
15
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
11
10
9
9
7
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
273
86
79
60
50
30
20
13
8
7
2
1
Supplier
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Catalog No. ABIN959855
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN731528 FACS IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 2
1 ABIN1804096 IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN731537 IHC (p) WB HRP Rabbit IgG AA 120-170 Log in to see Polyclonal 0
1 ABIN731530 IHC (p) WB Biotin Rabbit IgG AA 120-170 Log in to see Polyclonal 0
1 ABIN2963945 IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope AA 2-16
(89), (62), (18), (16), (14), (12), (10), (9), (8), (8), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(287), (121), (107), (18), (1), (1), (1)
Host Rabbit
(254), (24), (15)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(14), (11), (10), (9), (9), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(273), (86), (79), (60), (50), (30), (20), (13), (8), (7), (2), (1)
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kD pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53. Four isoforms of PUMA have been described due to alternative splicing. Is expected to recognize the canonical isofom 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%) Opossum (87%).
Purification Purified
Immunogen Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human PUMA alpha. Percent identity by BLAST analysis: Human, Gibbon, Mouse, Rat, Porcine (100%), Opossum (87%).

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Name/Gene ID: BBC3
Family: Apoptosis

Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA
Gene ID 27113
UniProt Q9BXH1
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Application Notes Approved: IHC-P (2.5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Human breast carcinoma. Western Blot: detects a band of approximately 23kD in K562 cell lysates.
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2 Binding Component 3 (BBC3) (AA 2-16) antibody (ABIN959855) Anti-BBC3 / PUMA antibody IHC staining of human colon. Immunohistochemistry of formal...