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|Antigen||Huntingtin Interacting Protein 1 (HIP1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|9 references available|
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Product Details anti-HIP1 AntibodyTarget Details HIP1 Application Details Handling References for anti-HIP1 antibody (ABIN152544) Images
|No Cross-Reactivity||Rat (Rattus)|
|Immunogen||Human 3'-HIP1 (~65 kDa)|
Target Details HIP1Product Details anti-HIP1 Antibody Application Details Handling References for anti-HIP1 antibody (ABIN152544) Images back to top
|Alternative Name||HIP1 (HIP1 Antibody Abstract)|
|Background||Gene Symbol: HIP1|
|Molecular Weight||Theoretical MW: 115 kDa|
|Pathways||Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-HIP1 Antibody Target Details HIP1 Handling References for anti-HIP1 antibody (ABIN152544) Images back to top
|Application Notes||Western Blot 1:10000, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-ParaffinThis HIP1 antibody is useful for Western blot, where a band at approximately 115 kDa is seen. Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation and Immunoprecipitation were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for HIP1 Antibody Western Blot Procedure
1. Run 25 µg/lane of protein (ie: HeLa whole cell extract) on a 10 % SDS-PAGE gel.
. Transfer the proteins to nitrocellulose.
. Block the membrane with a standard blocking buffer (5 % NFDM in TBST), overnight at 4 deg C.
. Dilute the anti-HIP1 in 1 % NFDM in TBST and incubate for 1hour at RT.
. Wash the membrane, 3x 5-10 minutes.
. Dilute the secondary antibody [anti-mouse IgG conjugated to HRP] in 1 % NFDM in TBST and incubate for 1hour at RT.
. Wash the membrane, 3x 5-10 minutes.
. Detect the protein-antibody complex via ECL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-HIP1 Antibody Target Details HIP1 Application Details References for anti-HIP1 antibody (ABIN152544) Images back to top
Buffer contains: 0.1 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-HIP1 antibody (ABIN152544)Product Details anti-HIP1 Antibody Target Details HIP1 Application Details Handling Images back to top
|Product cited in:||
Fontaine, Bauer, Lin, Poorfarahani, Ybe: "Replacement of charged and polar residues in the coiled-coiled interface of huntingtin-interacting protein 1 (HIP1) causes aggregation and cell death." in: FEBS letters, Vol. 586, Issue 19, pp. 3030-6, 2012 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
Barker, Benovic: "G protein-coupled receptor kinase 5 phosphorylation of hip regulates internalization of the chemokine receptor CXCR4." in: Biochemistry, Vol. 50, Issue 32, pp. 6933-41, 2011 (Sample species: Human). Further details: Western Blotting
Bradley, Holland, Liu, Thomas, Hyun, Ross: "Huntingtin interacting protein 1 is a novel brain tumor marker that associates with epidermal growth factor receptor." in: Cancer research, Vol. 67, Issue 8, pp. 3609-15, 2007 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Bradley, Smith, Hyun, Lucas, Li, Antonuk, Joshi, Jin, Ross: "Aberrant Huntingtin interacting protein 1 in lymphoid malignancies." in: Cancer research, Vol. 67, Issue 18, pp. 8923-31, 2007
Mills, Gaughan, Robson, Ross, McCracken, Kelly, Neal: "Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors." in: The Journal of cell biology, Vol. 170, Issue 2, pp. 191-200, 2005 (Sample species: Human). Further details: Chromatin Immunoprecipitation
Bradley, Oravecz-Wilson, Bougeard, Mizukami, Li, Munaco, Sreekumar, Corradetti, Chinnaiyan, Sanda, Ross: "Serum antibodies to huntingtin interacting protein-1: a new blood test for prostate cancer." in: Cancer research, Vol. 65, Issue 10, pp. 4126-33, 2005 Further details: Western Blotting
Hyun, Rao, Saint-Dic, Michael, Kumar, Bradley, Mizukami, Oravecz-Wilson, Ross: "HIP1 and HIP1r stabilize receptor tyrosine kinases and bind 3-phosphoinositides via epsin N-terminal homology domains." in: The Journal of biological chemistry, Vol. 279, Issue 14, pp. 14294-306, 2004 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence
Rao, Hyun, Kumar, Mizukami, Rubin, Lucas, Sanda, Ross: "Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival." in: The Journal of clinical investigation, Vol. 110, Issue 3, pp. 351-60, 2002
Rao, Chang, Kumar, Mizukami, Smithson, Bradley, Parlow, Ross: "Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors." in: Molecular and cellular biology, Vol. 21, Issue 22, pp. 7796-806, 2001 Further details: Immunocytochemistry,Western Blotting,Immunoprecipitation,Immunofluorescence
ImagesProduct Details anti-HIP1 Antibody Target Details HIP1 Application Details Handling References for anti-HIP1 antibody (ABIN152544) back to top