VEGF 165 Protein
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- Target
- VEGF 165
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
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- Comment
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Synonyms: Vascular endothelial growth factor A, VEGF, MVCD1, VPF
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA, 0.1 MM PMSF, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- VEGF 165
- Background
- Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- Molecular Weight
- 19.3 kDa (166aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_001020539
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