HVEM Protein (Fc Tag)
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- Target See all HVEM (TNFRSF14) Proteins
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Hi-5 Cells
- Purification tag / Conjugate
- This HVEM protein is labelled with Fc Tag.
- Application
- Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human TNFRSF14 / HVEM protein expressed in Hi-5 Insect cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to neutralize 0.25 ng/ml of hTNF&beta induced cytotoxicity on murine L929 cells. The expected ED50 for this effect is 1.3-1.9 ug/mL of HVEM-Fc.
- Top Product
- Discover our top product TNFRSF14 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- Alternative Name
- TNFRSF14 / HVEM (TNFRSF14 Products)
- Synonyms
- ATAR Protein, CD270 Protein, HVEA Protein, HVEM Protein, LIGHTR Protein, TR2 Protein, Atar Protein, HveA Protein, Hvem Protein, Tnfrs14 Protein, TNF receptor superfamily member 14 Protein, tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Protein, Tnfrsf14 Protein, TNFRSF14 Protein
- Target Type
- Viral Protein
- Background
- This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 41 kDa
- NCBI Accession
- NP_003811
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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