RAN Protein (full length) (rho-1D4 tag)

Catalog No. ABIN7126070

Product Details

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Protein Type: Recombinant

Protein Characteristics: full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This RAN protein is labelled with rho-1D4 tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), Functional Studies (Func)

Sequence: MAAQGEPPVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL LFHTSRGPIK FNVWDTAGQE KFGGLRDGYY IQAQSAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDVKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLSRRLTG DPNLEFVAMP ALAPPEVIMD PVLAMKYERD LEVAQATALP DEEDDL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO GTP-binding nuclear protein Ran Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RAN (RAN, Member RAS Oncogene Family (RAN))

Alternative Name: GTP-binding nuclear protein Ran (RAN Products)

Synonyms: ARA24 Protein, Gsp1 Protein, TC4 Protein, ran Protein, ara24 Protein, gsp1 Protein, ran-1 Protein, tc4 Protein, RAN Protein, RANP1 Protein, AAF30287 Protein, CG1404 Protein, Dmel\CG1404 Protein, Ran Protein, dran Protein, l(1)G0075 Protein, ran10A Protein, fc16b04 Protein, wu:fc16b04 Protein, RAN, member RAS oncogene family Protein, RAN, member RAS oncogene family S homeolog Protein, CG1404 gene product from transcript CG1404-RC Protein, RAN Protein, Ran Protein, ran.S Protein, ran Protein

Background: GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle. {ECO:0000256|RuleBase:RU363057}.

UniProt: G3IHE5

Pathways: Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus