GADD45A Protein (His tag)
Quick Overview for GADD45A Protein (His tag) (ABIN7319595)
Target
See all GADD45A ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This GADD45A protein is labelled with His tag.
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Purpose
- Recombinant Human GADD45A/DDIT-1 Protein (His Tag)
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Sequence
- Met1-Arg165
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Characteristics
- Recombinant Human GADD45A alpha is produced by our E.coli expression system and the target gene encoding Met1-Arg165 is expressed with a 6His tag at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (His tag)
custom-made GADD45A Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (His tag)custom-made GADD45A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) (AA 1-165) protein (GST tag)GADD45A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))
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Alternative Name
- GADD45A/DDIT-1
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Background
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Background: Growth Arrest and DNA Damage-Inducible Protein GADD45 α (GADD45A) is a member of the GADD45 family. GADD45A can be induced by UV irradiation, X-rays, growth arrest and alkylating agents, of which can be mediated by some kinases other than PKC. GADD45A can interact with MAPK14, GADD45GIP1, PCNA. In T-cells, GADD45A functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. GADD45A may affect PCNA interaction with some cell division protein kinase complexes, stimulating DNA excision repair in vitro and inhibits entry of cells into S phase.
Synonym: Growth Arrest and DNA Damage-Inducible Protein GADD45 Alpha, DNA Damage-Inducible Transcript 1 Protein, DDIT-1, GADD45A, DDIT1, GADD45
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Molecular Weight
- 20.5 kDa
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UniProt
- P24522
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Pathways
- p53 Signaling, Cell Division Cycle
Target
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