anti-Rat (Rattus) alpha Actinin 4 antibody for Western Blotting

Recommended alpha Actinin 4 Antibody (supplied by: Log in to see )

Antigen
Actinin, alpha 4 (ACTN4) Antibodies
  • fsgs
  • fsgs1
  • C77391
  • ACTININ-4
  • FSGS
  • FSGS1
  • wu:fb53f05
  • zgc:63508
  • actinin alpha 4
  • actinin, alpha 4
  • actinin alpha 4 S homeolog
  • actn4
  • ACTN4
  • Actn4
  • actn4.S
Epitope
N-Term
18
11
11
9
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
109
36
32
8
4
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
85
24
Clonality
Polyclonal
Conjugate
This alpha Actinin 4 antibody is un-conjugated
3
3
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
103
52
50
23
23
19
12
10
5
4
2
1
1
1
Options
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Catalog No. ABIN2775288
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1712315 ABIN2775289 WB Rabbit C-Term Log in to see Polyclonal 2
3.1712315 ABIN396311 ELISA IF IHC WB Mouse IgG2a kappa AA 592-702 Log in to see 4D10 5
3.1712315 ABIN4886421 IHC (p) WB Rabbit IgG AA 561-661 Log in to see Polyclonal 0
3.1712315 ABIN2855119 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.1712315 ABIN781950 EIA IF IHC (p) WB Mouse IgG2a Log in to see 4D10 0
3.1712315 ABIN4279711 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.1712315 ABIN2856884 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
3.1712315 ABIN2745506 IP IHC WB Rabbit AA 27-38, C-Term, N-Term Log in to see Polyclonal 0
3.1712315 ABIN629744 WB Rabbit N-Term Log in to see Polyclonal 0
3.1712315 ABIN6259756 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1712315 ABIN4279712 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.1712315 ABIN4279713 ICC IF WB Rabbit IgG N-Term Log in to see Polyclonal 0
3.1712315 ABIN2429555 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN204989 WB Rabbit IgG AA 201-250 Log in to see Polyclonal 0
1 ABIN626015 ELISA IF IHC IHC (p) WB Mouse IgG2a, kappa AA 592-702 Log in to see 4D10 0
1 ABIN215583 IHC IHC (fro) IHC (p) IP WB Rabbit IgG AA 28-38 Log in to see Polyclonal 0
1 ABIN1723628 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 592-702 Log in to see 4D10 0
1 ABIN5689869 IHC IP WB Rabbit AA 27-38 Log in to see Polyclonal 0
1 ABIN2746139 ELISA IHC IP WB FITC Rabbit IgG AA 140-190 Log in to see Polyclonal 0
1 ABIN2746138 ELISA IHC IP WB Rabbit IgG AA 140-190 Log in to see Polyclonal 0

General

Antigen Actinin, alpha 4 (ACTN4) Antibodies
Epitope N-Term
(18), (11), (11), (9), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(109), (36), (32), (8), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(85), (24)
Clonality
Polyclonal
Conjugate This alpha Actinin 4 antibody is un-conjugated
(3), (3), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(103), (52), (50), (23), (23), (19), (12), (10), (5), (4), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-alpha Actinin 4 Antibody

Target Details alpha Actinin 4 Application Details Handling References for anti-alpha Actinin 4 antibody (ABIN2775288) Images
Sequence LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM LDAEDIVNTA
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against ACTN4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN4
Plasmids, Primers & others

Target Details alpha Actinin 4

Product Details anti-alpha Actinin 4 Antibody Application Details Handling References for anti-alpha Actinin 4 antibody (ABIN2775288) Images back to top
Antigen
Alternative Name ACTN4 (ACTN4 Antibody Abstract)
Background Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN4 is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in its gene have been associated with focal and segmental glomerulosclerosis.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
Alias Symbols: DKFZp686K23158, FSGS, FSGS1, ACTININ-4
Protein Interaction Partner: MYOZ2, HUWE1, UBC, BMP7, ACTN3, SUMO2, SUMO3, GNB2, SUMO1, NEDD8, MDM2, HGS, SF3A3, VCP, CDKN2A, ACTN1, PDLIM1, UBD, IGSF8, ICAM1, CD81, SVIL, Fcho2, POT1, VCAM1, TXN, RPS27, MAPK7, KPNA2, ITGA4, FN1, ESR1, CDH1, TRAF3IP1, FMNL1, NOS3, ACTN2, UBASH3B, SHC
Protein Size: 911
Molecular Weight 100 kDa
Gene ID 81
NCBI Accession NM_004924, NP_004915
UniProt O43707
Pathways Proton Transport

Application Details

Product Details anti-alpha Actinin 4 Antibody Target Details alpha Actinin 4 Handling References for anti-alpha Actinin 4 antibody (ABIN2775288) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 911 AA

Restrictions For Research Use only

Handling

Product Details anti-alpha Actinin 4 Antibody Target Details alpha Actinin 4 Application Details References for anti-alpha Actinin 4 antibody (ABIN2775288) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-alpha Actinin 4 antibody (ABIN2775288)

Product Details anti-alpha Actinin 4 Antibody Target Details alpha Actinin 4 Application Details Handling Images back to top
Product cited in:

Wei, Gao, Du, Su, Xu: "Angiogenin enhances cell migration by regulating stress fiber assembly and focal adhesion dynamics." in: PLoS ONE, Vol. 6, Issue 12, pp. e28797, 2011

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006

Background publications

Kimura, Wakamatsu, Suzuki, Ota, Nishikawa, Yamashita, Yamamoto, Sekine, Tsuritani, Wakaguri, Ishii, Sugiyama, Saito, Isono, Irie, Kushida, Yoneyama, Otsuka, Kanda, Yokoi, Kondo, Wagatsuma, Murakawa et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006

Images

Product Details anti-alpha Actinin 4 Antibody Target Details alpha Actinin 4 Application Details Handling References for anti-alpha Actinin 4 antibody (ABIN2775288) back to top
Supplier Images
Western Blotting (WB) image for anti-Actinin, alpha 4 (ACTN4) (N-Term) antibody (ABIN2775288) WB Suggested Anti-ACTN4 Antibody Titration: 0.625ug/ml Positive Control: HCT116 ce...
Western Blotting (WB) image for anti-Actinin, alpha 4 (ACTN4) (N-Term) antibody (ABIN2775288) WB Suggested Anti-ACTN4
Antibody Titration: 0.625 µg/mL
Positive Contro...
Western Blotting (WB) image for anti-Actinin, alpha 4 (ACTN4) (N-Term) antibody (ABIN2775288) Lanes: Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate Lane2: 10 ug ACTN2-GFP trans...
Immunohistochemistry (IHC) image for anti-Actinin, alpha 4 (ACTN4) (N-Term) antibody (ABIN2775288) Sample Type : ACTNX-GFP transfected COS-7 cells Primary Antibody Dilution : 1:1000...