anti-Rat (Rattus) alpha Actinin 4 antibody for Western Blotting

Recommended alpha Actinin 4 Antibody (supplied by: Log in to see )

Antigen
Actinin, alpha 4 (ACTN4) Antibodies
  • fsgs
  • fsgs1
  • C77391
  • ACTININ-4
  • FSGS
  • FSGS1
  • wu:fb53f05
  • zgc:63508
  • actinin alpha 4
  • actinin, alpha 4
  • actinin alpha 4 S homeolog
  • actn4
  • ACTN4
  • Actn4
  • actn4.S
Epitope
C-Term
19
11
10
5
4
4
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
109
36
32
8
5
4
4
3
3
3
2
2
1
1
1
Host
Rabbit
85
24
Clonality
Polyclonal
Conjugate
This alpha Actinin 4 antibody is un-conjugated
3
3
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
103
53
50
22
22
19
12
9
5
4
2
1
1
1
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Catalog No. ABIN2855119
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
32.018665 ABIN2856884 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
20.018665 ABIN2745506 IP IHC WB Rabbit AA 27-38, C-Term, N-Term Log in to see Polyclonal 0
19.78973 ABIN396311 ELISA IF IHC WB Mouse IgG2a kappa AA 592-702 Log in to see 4D10 5
19.78973 ABIN781950 EIA IF IHC (p) WB Mouse IgG2a Log in to see 4D10 0
18.28973 ABIN2775288 IHC WB Rabbit N-Term Log in to see Polyclonal 3
17.018665 ABIN629744 WB Rabbit N-Term Log in to see Polyclonal 0
15.289729 ABIN2775289 WB Rabbit C-Term Log in to see Polyclonal 2
13.789729 ABIN4886421 IHC (p) WB Rabbit IgG AA 561-661 Log in to see Polyclonal 0
10.789729 ABIN2429555 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
9.292006 ABIN4279711 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
7.792006 ABIN4279712 ICC IF IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
7.7897296 ABIN6259756 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN626015 ELISA IF IHC IHC (p) WB Mouse IgG2a, kappa AA 592-702 Log in to see 4D10 0
4 ABIN204989 WB Rabbit IgG AA 201-250 Log in to see Polyclonal 0
4 ABIN1723628 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 592-702 Log in to see 4D10 0
4 ABIN2471154 IF IHC ELISA WB Mouse IgG2a kappa Log in to see 4D10 0
1.7920059 ABIN4279713 ICC IF WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN215583 IHC IHC (fro) IHC (p) IP WB Rabbit IgG AA 28-38 Log in to see Polyclonal 0
1 ABIN5689869 IHC IP WB Rabbit AA 27-38 Log in to see Polyclonal 0
1 ABIN2746139 ELISA IHC IP WB FITC Rabbit IgG AA 140-190 Log in to see Polyclonal 0

General

Antigen Actinin, alpha 4 (ACTN4) Antibodies
Epitope C-Term
(19), (11), (10), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(109), (36), (32), (8), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(85), (24)
Clonality
Polyclonal
Conjugate This alpha Actinin 4 antibody is un-conjugated
(3), (3), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(103), (53), (50), (22), (22), (19), (12), (9), (5), (4), (2), (1), (1), (1)
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Product Details anti-alpha Actinin 4 Antibody

Target Details alpha Actinin 4 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to alpha Actinin 4 (actinin, alpha 4)
alpha Actinin 4 antibody [C2C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human alpha Actinin 4. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details alpha Actinin 4

Product Details anti-alpha Actinin 4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name alpha Actinin 4 (ACTN4 Antibody Abstract)
Background Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Cellular Localization: Nucleus , Cytoplasm
Molecular Weight 105 kDa
Gene ID 81
Pathways Proton Transport

Application Details

Product Details anti-alpha Actinin 4 Antibody Target Details alpha Actinin 4 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , mouse brain , PC-12

Restrictions For Research Use only

Handling

Product Details anti-alpha Actinin 4 Antibody Target Details alpha Actinin 4 Application Details Images back to top
Format Liquid
Concentration 0.32 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-alpha Actinin 4 Antibody Target Details alpha Actinin 4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) WB Image alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by Western blo...
Immunohistochemistry (IHC) image for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) IHC-P Image alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein o...
Western Blotting (WB) image for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) WB Image alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by Western blo...
Western Blotting (WB) image for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE antibody dilu...
Immunohistochemistry (IHC) image for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) IHC-P Image Immunohistochemical analysis of paraffin-embedded A549 xenograft, using a...
Western Blotting (WB) image for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) WB Image alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by Western blo...
Immunofluorescence (IF) image for anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody (ABIN2855119) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...