anti-Human Metabotropic Glutamate Receptor 5 antibody for Enzyme Immunoassay

Recommended Metabotropic Glutamate Receptor 5 Antibody

Antigen
Glutamate Receptor, Metabotropic 5 (GRM5) Antibodies
  • GPRC1E
  • MGLUR5
  • mGlu5
  • mGluR5
  • mGlur5
  • 6430542K11Rik
  • AI850523
  • Glu5R
  • Gprc1e
  • mGluR5b
  • glutamate receptor, metabotropic 5
  • glutamate metabotropic receptor 5
  • GRM5
  • Grm5
Epitope
C-Term
82
70
15
14
11
7
4
3
3
2
2
2
2
1
Reactivity
Human
199
156
146
7
7
5
5
3
3
3
2
2
1
Host
Rabbit
109
91
4
2
Clonality
Polyclonal
Conjugate
This Metabotropic Glutamate Receptor 5 antibody is un-conjugated
9
7
7
6
6
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
165
113
83
69
58
37
36
22
13
4
4
2
2
1
Options

Available images

Catalog No. ABIN358930
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN317705 EIA IF IHC (p) WB Rabbit Polyclonal 0
1 ABIN1108229 EIA IHC (fro) WB Rabbit C-Term Polyclonal 0

General

Antigen Glutamate Receptor, Metabotropic 5 (GRM5) Antibodies
Epitope C-Term
(82), (70), (15), (14), (11), (7), (4), (3), (3), (2), (2), (2), (2), (1)
Reactivity Human
(199), (156), (146), (7), (7), (5), (5), (3), (3), (3), (2), (2), (1)
Host Rabbit
(109), (91), (4), (2)
Clonality
Polyclonal
Conjugate This Metabotropic Glutamate Receptor 5 antibody is un-conjugated
(9), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(165), (113), (83), (69), (58), (37), (36), (22), (13), (4), (4), (2), (2), (1)
Pubmed 5 references available
Supplier

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Target Details Metabotropic Glutamate Receptor 5 Application Details Handling References for anti-Metabotropic Glutamate Receptor 5 antibody (ABIN358930) Images
Specificity This antibody reacts to Metabotropic Glutamate Receptor 5 (GPRC1E).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GPRC1E.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details Metabotropic Glutamate Receptor 5

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Application Details Handling References for anti-Metabotropic Glutamate Receptor 5 antibody (ABIN358930) Images back to top
Antigen
Alternative Name mGluR5 / GRM5 (GRM5 Antibody Abstract)
Background L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 (also known as GPRC1E) and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. The activity of GRM5 is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current.Synonyms: GPRC1E, MGLUR5, Metabotropic glutamate receptor 5
Gene ID 2915, 9606
UniProt P41594
Pathways Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Handling References for anti-Metabotropic Glutamate Receptor 5 antibody (ABIN358930) Images back to top
Application Notes ELISA: 1/1,000. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Application Details References for anti-Metabotropic Glutamate Receptor 5 antibody (ABIN358930) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Metabotropic Glutamate Receptor 5 antibody (ABIN358930)

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Application Details Handling Images back to top
Background publications

Pacheco, Ciruela, Casadó, Mallol, Gallart, Lluis, Franco: "Group I metabotropic glutamate receptors mediate a dual role of glutamate in T cell activation." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33352-8, 2004

Anneser, Ince, Shaw, Borasio: "Differential expression of mGluR5 in human lumbosacral motoneurons." in: Neuroreport, Vol. 15, Issue 2, pp. 271-3, 2004

Uchino, Sakai, Kashiwagi, Shirai, Shinohara, Hirose, Iino, Yamamura, Saito: "Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC." in: The Journal of biological chemistry, Vol. 279, Issue 3, pp. 2254-61, 2004

Corti, Clarkson, Crepaldi, Sala, Xuereb, Ferraguti: "Gene structure of the human metabotropic glutamate receptor 5 and functional analysis of its multiple promoters in neuroblastoma and astroglioma cells." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 33105-19, 2003

Aronica, Gorter, Ijlst-Keizers, Rozemuller, Yankaya, Leenstra, Troost: "Expression and functional role of mGluR3 and mGluR5 in human astrocytes and glioma cells: opposite regulation of glutamate transporter proteins." in: The European journal of neuroscience, Vol. 17, Issue 10, pp. 2106-18, 2003

Images

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody Target Details Metabotropic Glutamate Receptor 5 Application Details Handling References for anti-Metabotropic Glutamate Receptor 5 antibody (ABIN358930) back to top
Supplier Images
Western Blotting (WB) image for anti-Glutamate Receptor, Metabotropic 5 (GRM5) (C-Term) antibody (ABIN358930) Western blot analysis of Metabotropic Glutamate Receptor 5 (GPRC1E) antibody (C-term)...