anti-Hamster GRB2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • grb2b
Reactivity
Cow (Bovine), Hamster, Human, Mouse (Murine), Rat (Rattus)
172
68
68
14
10
8
7
5
5
4
3
3
3
2
1
1
1
Host
Mouse
123
26
22
2
Clonality
Monoclonal
Conjugate
This GRB2 antibody is un-conjugated
9
5
4
3
3
3
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
158
84
74
26
14
6
5
5
4
1
1
Supplier
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Catalog No. ABIN364459
$ 727.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN481756 ELISA IHC IHC (p) WB Goat AA 206-217 Log in to see Polyclonal 0

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Reactivity Cow (Bovine), Hamster, Human, Mouse (Murine), Rat (Rattus)
(172), (68), (68), (14), (10), (8), (7), (5), (5), (4), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(123), (26), (22), (2)
Clonality
Monoclonal
Conjugate This GRB2 antibody is un-conjugated
(9), (5), (4), (3), (3), (3)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(158), (84), (74), (26), (14), (6), (5), (5), (4), (1), (1)
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes the N-terminal SH3 region of rat Grb2 at 23-25kD.
Purification Protein A purified
Immunogen Recombinant rat GRB2/Ash.

Type of Immunogen: Recombinant protein
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GRB2 (GRB2 Antibody Abstract)
Background Name/Gene ID: GRB2

Synonyms: GRB2, Abundant SRC homology, Adapter protein GRB2, ASH, EGFRBP-GRB2, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2, Grb3-3, HT027, NCKAP2
Gene ID 2885
UniProt P62993
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
Application Notes Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 mg/mL. Detects GRB2 (23-25kD) in RIPA lysates of non-stimulated human A431 carcinoma cells. Previous lots detected GRB2 in RIPA lysates of mouse 3T3 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells and bovine brain extract.
Comment

Target Species of Antibody: Rat

Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, 50 % glycerol.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibody (ABIN364459) Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with GRB2 antibody AB...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibody (ABIN364459) Human Spleen (formalin-fixed, paraffin-embedded) stained with GRB2 antibody ABIN36445...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibody (ABIN364459) Anti-GRB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed...