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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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1 | ABIN784591 | IHC (p) IP WB | Rabbit | IgG | AA 1035-1053 | Log in to see | Polyclonal | 0 | ||
1 | ABIN325544 | FACS IF IHC (p) IP WB | Rabbit | IgG | C-Term | Log in to see | 0 | |||
1 | ABIN1820653 | IP IHC WB | Rabbit | C-Term | Log in to see | Polyclonal | 0 | |||
1 | ABIN2467071 | IP IHC WB | Rabbit | IgG | AA 1035-1053 | Log in to see | Polyclonal | 0 |
General |
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Antigen | Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies |
Epitope | AA 1035-1053 Alternatives |
Reactivity | Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Xenopus laevis Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This PDGFRA antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-PDGFRA AntibodyTarget Details PDGFRA Application Details Handling Images |
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Brand | IHC-plus™ |
Specificity | Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish. |
Predicted Reactivity | Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%) Stickleback (95%) Zebrafish (89%). |
Purification | Immunoaffinity purified |
Immunogen |
Synthetic peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF (KLH-conjugated). Receptor alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%), Stickleback (95%), Zebrafish (89%). Type of Immunogen: Synthetic peptide - KLH conjugated |
Isotype | IgG |
Target Details PDGFRAProduct Details anti-PDGFRA Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | PDGFRA / PDGFR Alpha (PDGFRA Antibody Abstract) |
Background |
Name/Gene ID: PDGFRA Subfamily: PDGF Receptor Family: Protein Kinase Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha |
Gene ID | 5156 |
UniProt | P16234 |
Pathways | RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling |
Application DetailsProduct Details anti-PDGFRA Antibody Target Details PDGFRA Handling Images back to top |
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Application Notes |
Approved: IHC-P (1:100), IP, WB (1:250 - 1:1000) Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 μL immunoprecipitates PDGF Receptor a from 500 μg of 3T3/NIH RIPA lysate. |
Restrictions | For Research Use only |
HandlingProduct Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Images back to top |
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Format | Liquid |
Concentration | Lot specific |
Buffer | 0.1 M Tris-glycine, pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid repeat freeze-thaw cycles. |
Storage | 4 °C |
Storage Comment | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. |
ImagesProduct Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling back to top |
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Supplier Images |