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|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Goat, Hamster, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN967290) Images
|Cross-Reactivity||Rat (Rattus), Mouse (Murine), Hamster, Chicken, Rabbit, Goat, Dog (Canine), Monkey, Human|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Purified Cow Lens Vimentin|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN967290) Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well to organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Vimentin belongs to the type III category of IF proteins which are expressed in leukocytes, blood vessel endothelial cells, some epithelial cells, and mesenchymal cells. Vimentin is also expressed together with several other IF proteins during the early stages of development. Vimentin is exchanged for the tissue-specific intermediate filament type as differentiation proceeds. Vimentin migrates in SDS/PAGE as a ~57 kDa protein.|
|Molecular Weight||57 kDa|
|Pathways||Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN967290) Images back to top
Related Products: ABIN968535, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN967290) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4°C.|
References for anti-Vimentin antibody (ABIN967290)Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
|Product cited in:||
Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990
Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989
Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988
Broers, Carney, Klein Rot, Schaart, Lane, Vooijs, Ramaekers: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." in: Journal of cell science, Vol. 83, Issue 12, pp. 37-60, 1987
ImagesProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN967290) back to top