anti-Guinea Pig MAP2K3 antibody for Western Blotting

Recommended MAP2K3 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
  • MAPKK3
  • MEK3
  • MKK3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • AW212142
  • Prkmk3
  • mMKK3b
  • Mkk3
  • mitogen-activated protein kinase kinase 3
  • mitogen activated protein kinase kinase 3
  • MAP kinase activator XMEK3
  • MAP2K3
  • Map2k3
  • map2k3
Epitope
C-Term
38
28
18
13
12
11
11
10
10
7
7
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
313
167
152
55
39
5
4
4
3
2
1
1
1
1
Host
Rabbit
225
87
Clonality
Polyclonal
Conjugate
This MAP2K3 antibody is un-conjugated
7
7
6
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
270
126
99
85
66
26
17
12
10
7
3
1
1
1
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Pubmed
1 reference available

Available images

Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (C-Term) antibody (ABIN2777129)
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (C-Term) antibody (ABIN2777129)
Catalog No. ABIN2777129
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7.819988 ABIN2777128 IHC WB Rabbit N-Term Log in to see Polyclonal 1
4 ABIN320697 WB Rabbit IgG C-Term Log in to see Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies
Epitope C-Term
(38), (28), (18), (13), (12), (11), (11), (10), (10), (7), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(313), (167), (152), (55), (39), (5), (4), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(225), (87)
Clonality
Polyclonal
Conjugate This MAP2K3 antibody is un-conjugated
(7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(270), (126), (99), (85), (66), (26), (17), (12), (10), (7), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-MAP2K3 Antibody

Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 antibody (ABIN2777129) Images
Blocking Peptide Blocking peptide for this product available: ABIN979769
Sequence EEPSPQLPAD RFSPEFVDFT AQCLRKNPAE RMSYLELMEH PFFTLHKTKK
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against MAP2K3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human MAP2K3
Plasmids, Primers & others

Target Details MAP2K3

Product Details anti-MAP2K3 Antibody Application Details Handling References for anti-MAP2K3 antibody (ABIN2777129) Images back to top
Antigen
Alternative Name MAP2K3 (MAP2K3 Antibody Abstract)
Background MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.
Alias Symbols: MAPKK3, MEK3, MKK3, PRKMK3, SAPKK2, SAPKK-2
Protein Interaction Partner: UBC, EGFR, MAPK14, MAP3K5, ARRB1, PKN1, ALDOC, APP, LRRK2, MAPK8IP2, MAPK8IP3, MAPK8IP1, SPAG9, MAP3K2, MAP2K3, PPP2R4, MAP3K4, MAP3K3, TINF2, MYC, RPL13, TAOK2, TAOK1, DCTN1, DYRK1B, PLCB2, SMAD7, MAPK12, MAPK3, ELK1, MAP2K6, NPHS1,
Protein Size: 318
Molecular Weight 36 kDa
Gene ID 5606
NCBI Accession NM_002756, NP_002747
Pathways MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling References for anti-MAP2K3 antibody (ABIN2777129) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 318 AA
Restrictions For Research Use only

Handling

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details References for anti-MAP2K3 antibody (ABIN2777129) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MAP2K3 antibody (ABIN2777129)

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling Images back to top
Background publications

Prickett, Brautigan: "Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4217-27, 2007

Images

Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 antibody (ABIN2777129) back to top
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (C-Term) antibody (ABIN2777129) WB Suggested Anti-MAP2K3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Po...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (C-Term) antibody (ABIN2777129) MAP2K3 antibody - C-terminal region Formalin Fixed Paraffin Embedded Tissue:...