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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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7.736145 | ABIN2777128 | IHC WB | Rabbit | N-Term | Log in to see | Polyclonal | 1 | |||
4 | ABIN320697 | WB | Rabbit | IgG | C-Term | Log in to see | Polyclonal | 0 |
General |
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Antigen | Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) Antibodies |
Epitope | C-Term Alternatives |
Reactivity | Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This MAP2K3 antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
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1 reference available |
Supplier | Log in to see |
Product Details anti-MAP2K3 AntibodyTarget Details MAP2K3 Application Details Handling References for anti-MAP2K3 antibody (ABIN2777129) Images |
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Blocking Peptide | Blocking peptide for this product available: ABIN979769 |
Sequence | EEPSPQLPAD RFSPEFVDFT AQCLRKNPAE RMSYLELMEH PFFTLHKTKK |
Predicted Reactivity | Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%, Zebrafish: 100% |
Characteristics | This is a rabbit polyclonal antibody against MAP2K3. It was validated on Western Blot using a cell lysate as a positive control. |
Purification | Affinity Purified |
Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human MAP2K3 |
Plasmids, Primers & others | |
Target Details MAP2K3Product Details anti-MAP2K3 Antibody Application Details Handling References for anti-MAP2K3 antibody (ABIN2777129) Images back to top |
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Antigen | |
Alternative Name | MAP2K3 (MAP2K3 Antibody Abstract) |
Background |
MAP2K3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. Alias Symbols: MAPKK3, MEK3, MKK3, PRKMK3, SAPKK2, SAPKK-2 Protein Interaction Partner: UBC, EGFR, MAPK14, MAP3K5, ARRB1, PKN1, ALDOC, APP, LRRK2, MAPK8IP2, MAPK8IP3, MAPK8IP1, SPAG9, MAP3K2, MAP2K3, PPP2R4, MAP3K4, MAP3K3, TINF2, MYC, RPL13, TAOK2, TAOK1, DCTN1, DYRK1B, PLCB2, SMAD7, MAPK12, MAPK3, ELK1, MAP2K6, NPHS1, Protein Size: 318 |
Molecular Weight | 36 kDa |
Gene ID | 5606 |
NCBI Accession | NM_002756, NP_002747 |
Pathways | MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2 |
Application DetailsProduct Details anti-MAP2K3 Antibody Target Details MAP2K3 Handling References for anti-MAP2K3 antibody (ABIN2777129) Images back to top |
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Application Notes | Optimal working dilutions should be determined experimentally by the investigator. |
Comment |
Antigen size: 318 AA |
Restrictions | For Research Use only |
HandlingProduct Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details References for anti-MAP2K3 antibody (ABIN2777129) Images back to top |
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Format | Liquid |
Concentration | Lot specific |
Buffer | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid repeated freeze-thaw cycles. |
Storage | -20 °C |
Storage Comment | For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
References for anti-MAP2K3 antibody (ABIN2777129)Product Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling Images back to top |
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Background publications |
Prickett, Brautigan: "Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4217-27, 2007 |
ImagesProduct Details anti-MAP2K3 Antibody Target Details MAP2K3 Application Details Handling References for anti-MAP2K3 antibody (ABIN2777129) back to top |
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Supplier Images |