anti-Zebrafish AIFM1 antibody for Western Blotting

Recommended AIFM1 Antibody (supplied by: Log in to see )

Antigen
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
  • aif
  • pdcd8
  • PDCD8
  • AIFM1
  • AIF
  • PCD8
  • CG7263
  • DmAIF
  • Dmel\\CG7263
  • GB16024
  • DDBDRAFT_0187853
  • DDBDRAFT_0191137
  • DDB_0187853
  • DDB_0191137
  • AIFsh2
  • Hq
  • Pdcd8
  • mAIF
  • Aif
  • zgc:91994
  • CMTX4
  • COWCK
  • COXPD6
  • apoptosis-inducing factor, mitochondrion-associated, 1
  • Apoptosis inducing factor
  • apoptosis inducing factor
  • allograft inflammatory factor 1
  • apoptosis-inducing factor, mitochondrion-associated 1
  • aifm1
  • AIFM1
  • AIF
  • aif
  • AIF1
  • Aifm1
Epitope
C-Term
28
15
13
13
10
5
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish
178
77
74
19
10
9
6
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
139
32
6
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
156
62
50
35
26
19
13
11
10
6
5
3
3
2
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Catalog No. ABIN2778665
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1406112 ABIN926871 WB Rabbit Middle Region Log in to see Polyclonal

Similar anti-AIFM1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Sheep (Ovine) Cow (Bovine) Dog (Canine) Rabbit Pig (Porcine) Zebrafish Horse (Equine) Guinea Pig
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 3 Antibodies 3 Antibodies 1 Antibodies
Simple Western (SimWes) 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 156 Antibodies 64 Antibodies 61 Antibodies 10 Antibodies 11 Antibodies 3 Antibodies 8 Antibodies 2 Antibodies 3 Antibodies 4 Antibodies 1 Antibodies

General

Antigen Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Antibodies
Epitope C-Term
(28), (15), (13), (13), (10), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish
(178), (77), (74), (19), (10), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(139), (32), (6), (2)
Clonality
Polyclonal
Conjugate This AIFM1 antibody is un-conjugated
(4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(156), (62), (50), (35), (26), (19), (13), (11), (10), (6), (5), (3), (3), (2)
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Product Details anti-AIFM1 Antibody

Target Details AIFM1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN981498
Sequence VDSSLPTVGV FAKATAQDNP KSATEQSGTG IRSESETESE ASEITIPPST
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against PDCD8. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human PDCD8

Target Details AIFM1

Product Details anti-AIFM1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDCD8 (AIFM1 Antibody Abstract)
Background AIFM1 (PDCD8) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, AIFM1 induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.
Alias Symbols: AIF, PDCD8, COXPD6
Protein Interaction Partner: ISG15, STAU1, BAG6, UBC, NEDD8, MDM2, ASB12, RNF2, BMI1, SUZ12, PARK2, BAG3, ADRB2, HDAC11, OXSR1, ILK, PAN2, NOS2, MLH1, CFTR, ESR1, TST, FSCN1, CPOX, SHC1, FBXO6, TSC22D4, CAND1, COPS5, CUL1, CUL2, CUL3, CUL5, TONSL, BLNK, PIDD1, XIAP, AMFR, Tubb4b, Tub
Protein Size: 326
Molecular Weight 36 kDa
Gene ID 9131
NCBI Accession NM_145813, NP_665812
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration

Application Details

Product Details anti-AIFM1 Antibody Target Details AIFM1 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 326 AA

Restrictions For Research Use only

Handling

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-AIFM1 Antibody Target Details AIFM1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (C-Term) (ABIN2778665) WB Suggested Anti-PDCD8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (C-Term) (ABIN2778665) WB Suggested Anti-PDCD8
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.2500 <...