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|Antigen||PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1) Antibodies|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-PHLPP1 AntibodyTarget Details PHLPP1 Application Details Handling References for anti-PHLPP1 antibody (ABIN360194) Images
|Specificity||This antibody reacts to PHLPP1.|
|Purification||Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PHLPP1.|
Target Details PHLPP1Product Details anti-PHLPP1 Antibody Application Details Handling References for anti-PHLPP1 antibody (ABIN360194) Images back to top
|Alternative Name||PHLPP1 (PHLPP1 Antibody Abstract)|
|Background||PHLPP1 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes.Synonyms: KIAA0606, PH domain leucine-rich repeat-containing protein phosphatase 1, PHLPP, PLEKHE1, Pleckstrin homology domain-containing family E member 1, SCOP, Suprachiasmatic nucleus circadian oscillatory protein|
|Gene ID||23239, 9606|
|Pathways||PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PHLPP1 Antibody Target Details PHLPP1 Handling References for anti-PHLPP1 antibody (ABIN360194) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details References for anti-PHLPP1 antibody (ABIN360194) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PHLPP1 antibody (ABIN360194)Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details Handling Images back to top
Gao, Brognard, Newton: "The phosphatase PHLPP controls the cellular levels of protein kinase C." in: The Journal of biological chemistry, Vol. 283, Issue 10, pp. 6300-11, 2008
Brognard, Sierecki, Gao, Newton: "PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms." in: Molecular cell, Vol. 25, Issue 6, pp. 917-31, 2007
Gao, Furnari, Newton: "PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth." in: Molecular cell, Vol. 18, Issue 1, pp. 13-24, 2005
ImagesProduct Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details Handling References for anti-PHLPP1 antibody (ABIN360194) back to top