You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PHLPP1 antibody for Enzyme Immunoassay

Recommended PHLPP1 Antibody (supplied by: Log in to see )

Antigen
PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1) Antibodies
  • AI836256
  • mKIAA0606
  • PHLPP
  • Phlpp
  • PLEKHE1
  • Plekhe1
  • Scop
  • SCOP
Epitope
N-Term
12
7
3
2
1
1
1
1
1
1
Reactivity
Human
34
8
5
Host
Rabbit
34
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
27
10
7
6
4
4
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN360194
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000885838 ABIN1449987 EIA WB Rabbit IgG N-Term Log in to see Polyclonal 4

Top referenced anti-PHLPP1 antibody for Enzyme Immunoassay

Similar anti-PHLPP1 Antibodies

Application / Reactivity Human
ELISA 10 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 29 Antibodies

General

Antigen PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1) Antibodies
Epitope N-Term
(12), (7), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(34), (8), (5)
Host Rabbit
(34)
Clonality
Polyclonal
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(27), (10), (7), (6), (4), (4), (2), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-PHLPP1 Antibody

Target Details PHLPP1 Application Details Handling References for anti-PHLPP1 Antibody (ABIN360194) Images
Specificity This antibody reacts to PHLPP1.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PHLPP1.
Isotype Ig

Target Details PHLPP1

Product Details anti-PHLPP1 Antibody Application Details Handling References for anti-PHLPP1 Antibody (ABIN360194) Images back to top
Antigen
Alternative Name PHLPP1 (PHLPP1 Antibody Abstract)
Background PHLPP1 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes.Synonyms: KIAA0606, PH domain leucine-rich repeat-containing protein phosphatase 1, PHLPP, PLEKHE1, Pleckstrin homology domain-containing family E member 1, SCOP, Suprachiasmatic nucleus circadian oscillatory protein
Gene ID 23239, 9606
UniProt O60346
Pathways PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Handling References for anti-PHLPP1 Antibody (ABIN360194) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details References for anti-PHLPP1 Antibody (ABIN360194) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PHLPP1 Antibody (ABIN360194)

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details Handling Images back to top
Background publications

Gao, Brognard, Newton: "The phosphatase PHLPP controls the cellular levels of protein kinase C." in: The Journal of biological chemistry, Vol. 283, Issue 10, pp. 6300-11, 2008

Brognard, Sierecki, Gao, Newton: "PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms." in: Molecular cell, Vol. 25, Issue 6, pp. 917-31, 2007

Gao, Furnari, Newton: "PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth." in: Molecular cell, Vol. 18, Issue 1, pp. 13-24, 2005

Images

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details Handling References for anti-PHLPP1 Antibody (ABIN360194) back to top
Supplier Images
 image for anti-PHLPP1 antibody (PH Domain and Leucine Rich Repeat Protein Phosphatase 1) (N-Term) (ABIN360194) anti-PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1) (N-Term) antibody