The antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (Integrin beta2 chain). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Cross-Reactivity (Details)
Species reactivity (tested):Human
Purification
Affinity Chromatography on Protein A
Immunogen
Leucocytes of a patient suffering from a LGL-type leukaemia
Flow Cytometry. Immunoprecipitation. Western Blotting (2 μg/mL): Positive control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. Application note: Non-reducing conditions. SDS-PAGE (6 % separating gel, 4 % stacking gel). Immunohistochemistry (frozen section). Functional application: The antibody induces high-affinity conformation of LFA-1 complex. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch. General.
CD18, integrin b2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7