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HLA-DR antibody

HLA-DR Reactivity: Human FACS, WB, IP Host: Mouse Monoclonal MEM-12 unconjugated
Catalog No. ABIN125741
  • Target See all HLA-DR products
    HLA-DR
    Reactivity
    • 113
    • 21
    • 18
    • 17
    • 17
    • 14
    • 11
    • 8
    • 6
    • 4
    • 3
    • 3
    • 2
    Human
    Host
    • 98
    • 12
    • 6
    Mouse
    Clonality
    • 110
    • 5
    Monoclonal
    Conjugate
    • 38
    • 14
    • 14
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HLA-DR antibody is un-conjugated
    Application
    • 99
    • 31
    • 23
    • 18
    • 15
    • 9
    • 9
    • 8
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
    Specificity
    The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
    Cross-Reactivity (Details)
    Human
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    thymocyte membrane
    Clone
    MEM-12
    Isotype
    IgG1
  • Application Notes
    Flow cytometry: Recommended dilution: 2 μg/mL, positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line.
    Western blotting and Immunoprecipitation: Positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line, non-reducing conditions.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Horejsí, N?mec, Angelisová, Kristofová, Gorga, Hilgert: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).

  • Target
    HLA-DR
    Alternative Name
    HLA-DR (HLA-DR Products)
    Background
    HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
    Pathways
    Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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