E-cadherin antibody
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- Target See all E-cadherin (CDH1) Antibodies
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This E-cadherin antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- This antibody specifically recognizes E-cadherin at 124 kDa.
- Characteristics
- Synonyms: Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
- Purification
- The antibody was purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.
- Components
- incl. positive Control
- Immunogen
- Peptide conjugated to hemocyanin. Epitope: cytoplasmic domain.
- Clone
- 22F8
- Isotype
- IgG1
- Top Product
- Discover our top product CDH1 Primary Antibody
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- Application Notes
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from untreated A431 cells as positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- Positive control provided: Control cell lysate A 431 untreatedFormat: Lyophilized cell lysate from serum starved A431 cells. Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL H2O (15 min, RT).
- Buffer
- 2 x PBS / 0.09 % sodium azide / PEG and sucrose
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be storedat 4 °C up to 3 months. Avoid repeated freezing and thawing.
- Expiry Date
- 12 months
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- Target
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
- Alternative Name
- CD324 / Cadherin-1 (CDH1 Products)
- Synonyms
- Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody
- Background
- Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E-(epithelial), P-(placental), N-(neuronal), and M- muscle) cadherin. E-cadherin is expressed in most epithelial tissues.Synonyms: CAM 120/80, CDH1, CDHE, E-cadherin, Epithelial cadherin, UVO, Uvomorulin
- Gene ID
- 999
- UniProt
- P12830
- Pathways
- WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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