This antibody specifically recognizes STAT3 phosphorylated at Tyr 705. The antibody does not crossreact with the non-phosphorylated form of STAT3 nor with unrelated tyrosine-phosphorylated proteins.
Characteristics
Synonyms: STAT-3, Acute-phase response factor, APRF, Signal transducer and activator oftranscription 3
Purification
Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
Components
incl. positive Control
Immunogen
Phosphopeptide conjugated to KLHEpitope: Phosphotyrosine 705. . . A P pY L K. . .
KO Validated
STAT3
Reactivity: Human
WB, IHC, IP, IF
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Immunocytochemistry: 1-10 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide, PEG and Sucrose.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Aliquote and freeze in liquid nitrogen. Avoid repeated freezing and thawing. The antibody is stable at -80 °C for one year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Expiry Date
12 months
Target
STAT3
(Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
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Background
The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT3 is activated by tyrosine phosphorylation at residue Tyr 705 in cells treated with interleukin 6 or EGF. Activated STAT3 can bind to DNA either as homodimer or as heterodimer with STAT1.Synonyms: APRF, Acute-phase response factor, STAT-3, Signal transducer and activator of transcription 3