FOXO3 antibody (C-Term)

Catalog No. ABIN127307

Product Details anti-FOXO3 Antibody

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: This antibody reacts to FOXO3a. A ~80 kDa band should be detected.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to residues on the C-terminus of Human FOXO3a

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Positive Controls: Jurkat cell lysate and rat kidney.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 0.5 % BSA, 30 % glycerol and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FOXO3 / FKHRL1 (FOXO3 Products)

Synonyms: AF6q21 antibody, FKHRL1 antibody, FKHRL1P2 antibody, FOXO2 antibody, FOXO3A antibody, zgc:92176 antibody, wu:fb50g02 antibody, wu:fi33f07 antibody, Fkhrl1 antibody, Foxo3a antibody, FoxO3A antibody, 1110048B16Rik antibody, 2010203A17Rik antibody, C76856 antibody, Fkhr2 antibody, FOXO-3 antibody, FOXO3 antibody, foxo3a antibody, xfoxo3 antibody, foxO3 antibody, foxO antibody, fkhr antibody, foxo5 antibody, wu:fj65a12 antibody, zFkhr antibody, forkhead box O3 antibody, forkhead box O3A antibody, forkhead box O3 L homeolog antibody, forkhead box O3b antibody, FOXO3 antibody, foxo3a antibody, Foxo3 antibody, foxo3.L antibody, foxo3 antibody, foxO3 antibody, foxo3b antibody

Background: Mammalian forkhead members of the class O (FOXO) transcription factors, including FOXO1, FOXO3a, and FOXO4, are implicated in the regulation of a variety of cellular processes, including the cell cycle, apoptosis, DNA repair, stress resistance, and metabolism. FOXO proteins are negatively regulated by the phosphatidylinositol 3-kinase-Akt signaling pathway, which is activated by growth factors and cytokines. Recent studies indicate that the activities of FOXO proteins are also regulated by oxidative stress, which induces their phosphorylation, translocation to the nucleus, and acetylation-deacetylation. Similar to the tumor suppressor p53, FOXO is activated by stress and induces the expression of genes that contribute to cellcycle arrest, suggesting that it also functions as a tumor suppressor.Synonyms: AF6q21 protein, FOXO3A, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1

Gene ID: 2309

NCBI Accession: NP_001446

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis