FOXO3 antibody (pSer253)
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- Target See all FOXO3 Antibodies
- FOXO3 (Forkhead Box O3 (FOXO3))
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Binding Specificity
- pSer253
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FOXO3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificity
- The antibody detects endogenous level of FKHRL1 only when phosphorylated at serine 253.
- Immunogen
- Peptide sequence around phosphorylation site of pSer253 (A-V-S (p) -M-D) derived from Human FKHRL1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Top Product
- Discover our top product FOXO3 Primary Antibody
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- Application Notes
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C
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Over-expression of heat shock factor 1 phenocopies the effect of chronic inhibition of TOR by rapamycin and is sufficient to ameliorate Alzheimer's-like deficits in mice modeling the disease." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 880-93, (2013) (PubMed).
: "Activation of Akt/FoxO signaling pathway contributes to induction of neuroprotection against transient global cerebral ischemia by hypoxic pre-conditioning in adult rats." in: Journal of neurochemistry, Vol. 114, Issue 3, pp. 897-908, (2010) (PubMed).
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Over-expression of heat shock factor 1 phenocopies the effect of chronic inhibition of TOR by rapamycin and is sufficient to ameliorate Alzheimer's-like deficits in mice modeling the disease." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 880-93, (2013) (PubMed).
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- Target
- FOXO3 (Forkhead Box O3 (FOXO3))
- Alternative Name
- FKHRL1 (FOXO3 Products)
- Synonyms
- AF6q21 antibody, FKHRL1 antibody, FKHRL1P2 antibody, FOXO2 antibody, FOXO3A antibody, zgc:92176 antibody, wu:fb50g02 antibody, wu:fi33f07 antibody, Fkhrl1 antibody, Foxo3a antibody, FoxO3A antibody, 1110048B16Rik antibody, 2010203A17Rik antibody, C76856 antibody, Fkhr2 antibody, FOXO-3 antibody, FOXO3 antibody, foxo3a antibody, xfoxo3 antibody, foxO3 antibody, foxO antibody, fkhr antibody, foxo5 antibody, wu:fj65a12 antibody, zFkhr antibody, forkhead box O3 antibody, forkhead box O3A antibody, forkhead box O3 L homeolog antibody, forkhead box O3b antibody, FOXO3 antibody, foxo3a antibody, Foxo3 antibody, foxo3.L antibody, foxo3 antibody, foxO3 antibody, foxo3b antibody
- Background
- Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
- Molecular Weight
- 97 kDa
- NCBI Accession
- NP_001446, NM_001455
- UniProt
- O43524
- Pathways
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis
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