AKT3 antibody

Catalog No. ABIN1892292

Product Details anti-AKT3 Antibody

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100 % identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.

Type of Immunogen: Synthetic peptide

Clone: 9A5-H9-G7

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), IHC (20 μg/mL), WB (1:500 - 1:2000)

Usage: Anti-AKT3 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, sterile filtered

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details for AKT3

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Alternative Name: AKT3 (AKT3 Products)

Synonyms: MPPH antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AI851531 antibody, D930002M15Rik antibody, Nmf350 antibody, Pkbg antibody, AKT serine/threonine kinase 3 antibody, thymoma viral proto-oncogene 3 antibody, v-akt murine thymoma viral oncogene homolog 3b antibody, AKT3 antibody, Akt3 antibody, akt3b antibody

Background: Name/Gene ID: AKT3
Subfamily: RAC/AKT
Family: Protein Kinase

Synonyms: AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kinase Akt-3, RAC-gamma, MPPH, PKB-GAMMA, PKBG, Protein kinase B gamma, RAC-PK-gamma, STK-2

Gene ID: 10000

Pathways: PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling