HLA-DR antibody (APC)
(1 reference)-
- Target See all HLA-DR products
- HLA-DR
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HLA-DR antibody is conjugated to APC
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Application
- Flow Cytometry (FACS)
- Specificity
- The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
- Cross-Reactivity (Details)
- Human
- Purification
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- thymocyte membrane
- Clone
- MEM-12
- Isotype
- IgG1
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anti-HLA-DR (HLA-DR) antibody (FITC)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 FITC
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal TAL 1B5 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human FACS, WB, IP Host: Mouse Monoclonal MEM-12 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC Host: Mouse Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PE)HLA-DR Reactivity: Human, Dog, Non-Human Primate FACS Host: Mouse Monoclonal L243 PE
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human FACS, WB, IHC, IF, ISt, StM Host: Mouse Monoclonal SPM289 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human FACS, IP Host: Mouse Monoclonal HL-39 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC, Func Host: Mouse Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PE)HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 PE
anti-HLA-DR (HLA-DR) antibody (PerCP)HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 PerCP
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- Application Notes
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
- Comment
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The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Restrictions
- For Research Use only
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- Reconstitution
- No reconstitution is necessary.
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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-
: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).
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: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).
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- Target
- HLA-DR
- Alternative Name
- HLA-DR (HLA-DR Products)
- Background
- HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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