E-cadherin antibody (Cytoplasmic Domain)

Catalog No. ABIN198431

Product Details anti-E-cadherin Antibody

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Specific for E-cadherin but does show some minor cross-reactivity with P-cadherin. Reacts with human E-Cadherin protein. Reactivity with other species has not been tested.

Purification: Protein A purified

Immunogen: The cytoplasmic domain of human E-cadherin expressed as a fusion protein with MBP.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (2 - 10 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Western Blot: 1 μg/mL. Immunoprecipitation: 2-5 μg. Immunohistochemistry (formalin-fixed., paraffin embedded sections require a Heat Induced Epitope Retrieval (HIER) step prior to staining): 2-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1 / E Cadherin (CDH1 Products)

Synonyms: Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

Background: Name/Gene ID: CDH1
Family: Cadherin

Synonyms: CDH1, CD324, CDHE, Cell-CAM 120/80, E Cadherin, E-Cadherin, ECAD, Epithelial cadherin, Arc-1, UVO, Cadherin-1, CAM 120/80, CD324 antigen, LCAM, Uvomorulin

Gene ID: 999

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation