MDM2 antibody (AA 26-169)

Catalog No. ABIN216012

Product Details anti-MDM2 Antibody

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Binding Specificity: AA 26-169

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes human MDM2 protein. Does not react with mouse MDM2. Epitope recognized by this antibody is located within amino acids 26-169 of the human protein.

Purification: Protein A purified

Immunogen: A synthetic peptide derived from human MDM2 protein (1).

Type of Immunogen: Synthetic peptide

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: IF (1 - 5 μg/mL), IHC, IHC-Fr (1 - 5 μg/mL), IHC-P (1 - 5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Immunofluorescence: 1-5 μg/mL. Western Blot: 0.5-2 μg/mL (chemiluminescence detection). Immunohistochemistry (frozen): 1-5 μg/mL. Immunohistochemistry (formalin-fixed, paraffin-embedded): 1-5 μg/mL. Staining requires heat induced epitope retrieval (HIER) pretreatment. Immunoprecipitation: 1 μg/sample (autoradiographic detection). Positive control: OsA-CL, MCF-7 and HeLa cell lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide, 1 mg/mL BSA, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2 (MDM2 Products)

Synonyms: ACTFS antibody, HDMX antibody, hdm2 antibody, zgc:109834 antibody, 1700007J15Rik antibody, AA415488 antibody, Mdm-2 antibody, MDM2 proto-oncogene antibody, MDM2 proto-oncogene S homeolog antibody, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antibody, MDM2 oncogene, E3 ubiquitin protein ligase antibody, transformed mouse 3T3 cell double minute 2 antibody, MDM2 antibody, mdm2.S antibody, Mdm2 antibody, mdm2 antibody

Background: Name/Gene ID: MDM2

Synonyms: MDM2, ACTFS, Double minute 2 protein, Hdm2, MDM2 variant FB30, HDMX, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2 variant FB28

Gene ID: 4193

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway