This antibody is expected to recognize all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).
Purification
STAT3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
STAT3 antibody was raised against a 13 amino acid synthetic peptide near the internal region of STAT3.
KO Validated
STAT3
Reactivity: Human
WB, IHC, IP, IF
Host: Rabbit
Monoclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 90 kDa band observed in human heart lysates (calculated MW of 88.1 kDa according to NP_644805.1 and of NP_003141.2). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
STAT3
(Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))