STAT3 antibody (phosphorylated)

Catalog No. ABIN2747236

Product Details anti-STAT3 Antibody

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Binding Specificity: AA 700-800, phosphorylated

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: The antibody labels a strong band of PSTAT3 at 92 kDa in ABIN1741665 samples and in other cancer cell lines.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Does not cross react to any other cellular protein.

Characteristics: Phospho-specific STAT3 selective antibodies were generated against a peptide taken from the human protein. The PSTAT3-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The phospho-specific STAT3-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides PSTAT3 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for PSTAT3 antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: Phosphorylated synthetic peptide taken within amino acid region 700-800 on human STAT3 protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: APRF, HIES, Acute phase response factor, APRF, FLJ20882 Signal transducer and activator of transcription 3 antibody and 1110034 Rik antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55-0.75 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3 (STAT3 Products)

Synonyms: 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody

Background: Signal transducers and activators of transcription (STATs) are a family of 7 transcription factors that form part of the (Janus Kinase) JAK-STAT signaling cascade. This cascade is the basis of the signal transduction mechanism for many cytokine receptors. STATs translocate to the nucleus once activated by phosphorylation by JAKs. Many factors regulate the JAK-STAT pathway including STAT dephosphorylation by phosphatases, altered nuclear import-export dynamics of STAT, and STAT gene activation antagonists such as SOCS (suppressors of cytokine signaling) and PIAS (Prot. Inhibitors of Activated STATs, 1). The STAT3 gene is part of a family of the STAT proteins that carry out a dual function: signal transduction and activation of transcription. STAT3 is widely expressed and becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to a variety of stimuli such as EGF, IL-6, PDGF, IL- 2 and G-CSF. STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a key role in many cellular processes such as cell growth and apoptosis. Abnormal STAT3 protein activation is identified in many cancers of the breast, prostate, and pancreas, as well as cancers of blood-forming cells (leukemia and lymphoma,. Phosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies, monospecific for a target protein that is phosphorylated on a specific tyrosine, threonine, or serine residue. STAT3 is activated by phosphorylation on Ser-727, which leads to dimer formation, nuclear translocation, and regulation of gene expression. Transcriptional activation is regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways. Mutations in STAT3 cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) also known as hyper-IgE syndrome or Job syndrome. The human Phospho-specific STAT3 gene maps to chromosome 17q21.31 and encodes a 512 amino acid protein with phosphorylation occurring at Serine 727.

NCBI Accession: NP_644805

UniProt: P40763

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor