Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibodydetects V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3). Family: Protein Kinase Subfamily: RAC/AKT
Characteristics
Synonyms: RAC-PK-gamma, Protein kinase B gamma, PKBG, STK-2, RAC-gammaserine/threonine-protein kinase
Purification
Protein G Chromatography
Immunogen
Synthetic peptide corresponding to amino acid residues 119-136 of human Akt3AA Sequence: CSPTSQIDNIGEEEMDASRemarks: GenBank no. gi|4574744|gb|AAD24196. 1|AF135794_1. This sequence is identicalin human, mouse, rat, cow, dog, and chicken.
AKT3
Reactivity: Human
WB, ELISA, IF
Host: Mouse
Monoclonal
6E10
unconjugated
Application Notes
Western blot (1 μg/mL - 2 μg/mL). Immunohistochemistry on paraffin sections (10 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS, 0.05 % Sodium Azide, 0.05 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 - -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein. Akt/PKB phosphorylation is critical for it activity.Synonyms: PKBG, Protein kinase B gamma, RAC-PK-gamma, RAC-gamma serine/threonine-protein kinase, STK-2