AKT3 antibody (AA 119-136)

Catalog No. ABIN302246

Product Details anti-AKT3 Antibody

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Binding Specificity: AA 119-136

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibodydetects V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3). Family: Protein Kinase Subfamily: RAC/AKT

Characteristics: Synonyms: RAC-PK-gamma, Protein kinase B gamma, PKBG, STK-2, RAC-gammaserine/threonine-protein kinase

Purification: Protein G Chromatography

Immunogen: Synthetic peptide corresponding to amino acid residues 119-136 of human Akt3AA Sequence: CSPTSQIDNIGEEEMDASRemarks: GenBank no. gi|4574744|gb|AAD24196. 1|AF135794_1. This sequence is identicalin human, mouse, rat, cow, dog, and chicken.

Clone: 66C1247-1

Isotype: IgG1

Application Details

Application Notes: Western blot (1 μg/mL - 2 μg/mL). Immunohistochemistry on paraffin sections (10 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, 0.05 % Sodium Azide, 0.05 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 - -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for AKT3

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Alternative Name: AKT3 / PKB gamma (AKT3 Products)

Synonyms: MPPH antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AI851531 antibody, D930002M15Rik antibody, Nmf350 antibody, Pkbg antibody, AKT serine/threonine kinase 3 antibody, thymoma viral proto-oncogene 3 antibody, v-akt murine thymoma viral oncogene homolog 3b antibody, AKT3 antibody, Akt3 antibody, akt3b antibody

Background: Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein. Akt/PKB phosphorylation is critical for it activity.Synonyms: PKBG, Protein kinase B gamma, RAC-PK-gamma, RAC-gamma serine/threonine-protein kinase, STK-2

Gene ID: 10000

UniProt: Q9Y243

Pathways: PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling