Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This Mdm2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-424 amino acids from the C-terminal region of human Mdm2.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid freeze-thaw cycles.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date
6 months
Duan, Ma, Huang, DAmore, Zhang, Lei et al.: "The Clustered, Regularly Interspaced, Short Palindromic Repeats-associated Endonuclease 9 (CRISPR/Cas9)-created MDM2 T309G Mutation Enhances Vitreous-induced Expression of MDM2 and Proliferation and ..." in: The Journal of biological chemistry, Vol. 291, Issue 31, pp. 16339-47, (2017) (PubMed).
Zhou, Duan, Ma, Wu, Hu, Chen, Chee, Cui, Samad, Matsubara, Mukai, DAmore, Lei: "Introduction of the MDM2 T309G Mutation in Primary Human Retinal Epithelial Cells Enhances Experimental Proliferative Vitreoretinopathy." in: Investigative ophthalmology & visual science, Vol. 58, Issue 12, pp. 5361-5367, (2017) (PubMed).
Mokhtarzadeh, Parhiz, Hashemi, Abnous, Ramezani: "P53-Derived peptides conjugation to PEI: an approach to producing versatile and highly efficient targeted gene delivery carriers into cancer cells." in: Expert opinion on drug delivery, Vol. 13, Issue 4, pp. 477-91, (2016) (PubMed).
Guo, Yang, Yang, Linghu, Zhan, Brock, Herman, Zhang, Guo: "RASSF10 suppresses colorectal cancer growth by activating P53 signaling and sensitizes colorectal cancer cell to docetaxel." in: Oncotarget, Vol. 6, Issue 6, pp. 4202-13, (2015) (PubMed).
Stevenson, Sparks, Allende-Vega, Xirodimas, Lane, Saville: "The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2." in: The EMBO journal, Vol. 26, Issue 4, pp. 976-86, (2007) (PubMed).
Target
MDM2
(Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.