NCF4 antibody (AA 228-238)

Catalog No. ABIN462290

Product Details anti-NCF4 Antibody

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Binding Specificity: AA 228-238

Reactivity: Human, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This NCF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NCF4. This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%) Hamster, Panda, Dog, Bovine, Pig (91%) Drosophila, Mosquito, Sea anemone, Ant, Water flea, Beetle, Tick (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KDFPEEDDPTN from an internal region of human NCF4 (NP_000622.2, NP_038202.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Horse (100%), Hamster, Panda, Dog, Bovine, Pig (91%), Drosophila, Mosquito, Sea anemone, Ant, Water flea, Beetle, Tick (82%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.3 - 13 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Alternative Name: P40PHOX / NCF4 (NCF4 Products)

Synonyms: p40phox antibody, AI451400 antibody, NCF antibody, P40PHOX antibody, SH3PXD4 antibody, neutrophil cytosolic factor 4 antibody, ncf4 antibody, Ncf4 antibody, NCF4 antibody

Background: Name/Gene ID: NCF4

Synonyms: NCF4, SH3PXD4, NCF, Neutrophil cytosol factor 4, p40-phox, p40PHOX, NCF-4

Gene ID: 4689

NCBI Accession: NP_000622, NP_038202

UniProt: Q15080