E-cadherin antibody

Catalog No. ABIN490144

Product Details anti-E-cadherin Antibody

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody 67A4 recognizes CD324 / E-cadherin, an approximately 100 kD epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms. HLDA VIII.

Purification: Protein A purified

Immunogen: T-47D cells.

Type of Immunogen: Cells

Clone: 67A4

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-Fr, IP, WB

Usage: Flow Cytometry: Application note - Tested on cell lines CACO-2 and HT-29. In this case the recommended concentration is 5-10 μg/mL per 1 million cells/mL. Immunohistochemistry (frozen sections): Recommended dilution - 4-8 μg/mL. Positive tissue - tonsil.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide,  pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1 / E Cadherin (CDH1 Products)

Synonyms: Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

Background: Name/Gene ID: CDH1
Family: Cadherin

Synonyms: CDH1, CD324, CDHE, Cell-CAM 120/80, E Cadherin, E-Cadherin, ECAD, Epithelial cadherin, Arc-1, UVO, Cadherin-1, CAM 120/80, CD324 antigen, LCAM, Uvomorulin

Gene ID: 999

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation