HRAS antibody (C-Term)

Catalog No. ABIN5518760

Product Details anti-HRAS Antibody

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Binding Specificity: AA 111-137, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRAS antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for GTPase Hras(HRAS) detection. Tested with WB in Human,Mouse,Rat.

Sequence: MVLVGNKCDL AARTVESRQA QDLARSY

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for GTPase Hras(HRAS) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: Harvey rat sarcoma viral oncogene homolog
Protein Name: GTPase Hras

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GTPase HRAS (111-137aa MVLVGNKCDLAARTVESRQAQDLARSY), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: HRAS (HRAS Products)

Synonyms: C-BAS/HAS antibody, C-H-RAS antibody, C-HA-RAS1 antibody, CTLO antibody, H-RASIDX antibody, HAMSV antibody, HRAS1 antibody, K-RAS antibody, N-RAS antibody, RASH1 antibody, hras antibody, zgc:110250 antibody, HRAS antibody, H-RAS antibody, c-H-ras antibody, H-Ras antibody, K-Ras antibody, hras1 antibody, rash1 antibody, ras antibody, N-Ras antibody, c-bas/has antibody, H-ras antibody, Ha-ras antibody, Harvey-ras antibody, Hras-1 antibody, Kras2 antibody, c-Ha-ras antibody, c-rasHa antibody, hrasl antibody, zgc:110734 antibody, HRas proto-oncogene, GTPase antibody, v-Ha-ras Harvey rat sarcoma viral oncogene homolog a antibody, neuroblastoma RAS viral (v-ras) oncogene homolog pseudogene antibody, Harvey rat sarcoma viral oncogene homolog L homeolog antibody, Harvey rat sarcoma viral oncogene homolog antibody, Harvey rat sarcoma virus oncogene antibody, NRAS proto-oncogene, GTPase antibody, -Ha-ras Harvey rat sarcoma viral oncogene homolog b antibody, HRAS antibody, hrasa antibody, LOC733587 antibody, Hras antibody, hras.L antibody, hras antibody, NRAS antibody, hrasb antibody

Background: GTPase HRas, also known as transforming protein p21, is an enzyme that in humans is encoded by the HRAS gene. This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

Synonyms: C HA RAS1 | c-H-ras | GTPase HRAS | H RASIDX | Ha-Ras | HRAS1 | H-Ras-1 | K ras | N ras | P01112 | p19 H RasIDX protein | p21ras | RASH

Gene ID: 3265

UniProt: P01112

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling