alpha Adaptin antibody (AA 706-727)
-
- Target See all alpha Adaptin (AP2A1) Antibodies
- alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
-
Binding Specificity
- AA 706-727
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This alpha Adaptin antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA
- Purpose
- AP2A1 (aa706-727)
- Sequence
- ELEPPAPESP MALLADPAPA AD
- Specificity
- This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
- Cross-Reactivity
- Cow, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
-
-
- Application Notes
-
Western Blot: Approx 110 kDa band observed in Human Brain (Frontal cortex) lysates (calculated MW of 108 kDa according to NP_055018.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
-
Quantitative proteomics reveals reduction of endocytic machinery components in gliomas." in: EBioMedicine, Vol. 46, pp. 32-41, (2020) (PubMed).
: "
-
Quantitative proteomics reveals reduction of endocytic machinery components in gliomas." in: EBioMedicine, Vol. 46, pp. 32-41, (2020) (PubMed).
-
- Target
- alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
- Alternative Name
- AP2A1 (AP2A1 Products)
- Synonyms
- ADTAA antibody, AP2-ALPHA antibody, CLAPA1 antibody, adtaa antibody, clapa1 antibody, ap2-alpha antibody, AP2A1 antibody, Adtaa antibody, adaptor related protein complex 2 alpha 1 subunit antibody, adaptor-related protein complex 2, alpha 1 subunit antibody, AP-2 complex subunit alpha-1 antibody, AP2A1 antibody, ap2a1 antibody, Ap2a1 antibody, EDI_093420 antibody, EDI_259990 antibody
- Background
- AP2A1, adaptor-related protein complex 2, alpha 1 subunit, ADTAA, AP2-ALPHA, CLAPA1, 100 kDa coated vesicle protein A, AP-2 complex subunit alpha-1, adapter-related protein complex 2 alpha-1 subunit, adaptin, alpha A, adaptor protein complex AP-2 subunit
- Gene ID
- 160, 11771, 308578
- NCBI Accession
- NP_055018
- Pathways
- Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
-