alpha Adaptin antibody (Internal Region)

Catalog No. ABIN5687656

Product Details anti-alpha Adaptin Antibody

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha Adaptin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.

Purification: AP2A1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: AP2A1 Antibody was raised against synthetic peptide ELEPPAPESPMALLADPAPAAD found at an internal region of AP2A1.

Application Details

Application Notes: ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 110  kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108  kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.0 Immunocytochemistry:HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: AP2A1 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Minimize freezing and thawing.

Target Details for alpha Adaptin

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Alternative Name: AP2A1 (AP2A1 Products)

Synonyms: ADTAA antibody, AP2-ALPHA antibody, CLAPA1 antibody, adtaa antibody, clapa1 antibody, ap2-alpha antibody, AP2A1 antibody, Adtaa antibody, adaptor related protein complex 2 alpha 1 subunit antibody, adaptor-related protein complex 2, alpha 1 subunit antibody, AP-2 complex subunit alpha-1 antibody, AP2A1 antibody, ap2a1 antibody, Ap2a1 antibody, EDI_093420 antibody, EDI_259990 antibody

Molecular Weight: Approx 110kDa

Gene ID: 160

NCBI Accession: NP_055018

UniProt: O95782

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation