MDM2 antibody (pSer166)

Catalog No. ABIN5689770

Product Details anti-MDM2 Antibody

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Binding Specificity: pSer166

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: This antibody detects endogenous level of MDM2 only when phosphorylated at serine 166.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: MDM2 (phospho-Ser166) antibody was raised against a peptide sequence around phosphorylation site of Serine 166 (A-I-S (p) -E-T) derived from Human MDM2.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2 (MDM2 Products)

Synonyms: ACTFS antibody, HDMX antibody, hdm2 antibody, zgc:109834 antibody, 1700007J15Rik antibody, AA415488 antibody, Mdm-2 antibody, MDM2 proto-oncogene antibody, MDM2 proto-oncogene S homeolog antibody, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antibody, MDM2 oncogene, E3 ubiquitin protein ligase antibody, transformed mouse 3T3 cell double minute 2 antibody, MDM2 antibody, mdm2.S antibody, Mdm2 antibody, mdm2 antibody

Background: This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

Molecular Weight: 90 kDa

Gene ID: 4193

NCBI Accession: NP_002383

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway