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alpha Adaptin antibody (Internal Region)

AP2A1 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6259893
  • Target See all alpha Adaptin (AP2A1) Antibodies
    alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
    Binding Specificity
    • 8
    • 8
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 45
    • 15
    • 12
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 35
    • 6
    • 4
    Rabbit
    Clonality
    • 38
    • 7
    Polyclonal
    Conjugate
    • 22
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha Adaptin antibody is un-conjugated
    Application
    • 33
    • 22
    • 21
    • 8
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC)
    Specificity
    Alpha Adaptin Antibody detects endogenous levels of total alpha Adaptin.
    Predicted Reactivity
    Pig,Horse,Sheep,Rabbit
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human alpha Adaptin, corresponding to a region within the internal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product AP2A1 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))
    Alternative Name
    AP2A1 (AP2A1 Products)
    Synonyms
    ADTAA antibody, AP2-ALPHA antibody, CLAPA1 antibody, adtaa antibody, clapa1 antibody, ap2-alpha antibody, AP2A1 antibody, Adtaa antibody, adaptor related protein complex 2 alpha 1 subunit antibody, adaptor-related protein complex 2, alpha 1 subunit antibody, AP-2 complex subunit alpha-1 antibody, AP2A1 antibody, ap2a1 antibody, Ap2a1 antibody, EDI_093420 antibody, EDI_259990 antibody
    Background

    Description: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).

    Gene: AP2A1

    Molecular Weight
    108 kDa
    Gene ID
    160
    UniProt
    O95782
    Pathways
    Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation
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