GCKR antibody (Internal Region)

Catalog No. ABIN6261914

Product Details anti-GCKR Antibody

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCKR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: GCKR Antibody detects endogenous levels of total GCKR.

Predicted Reactivity: Pig,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GCKR, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GCKR

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Alternative Name: GCKR (GCKR Products)

Synonyms: FGQTL5 antibody, GKRP antibody, GLRE antibody, gckr antibody, glucokinase regulator antibody, glucokinase regulatory protein antibody, glucokinase regulator L homeolog antibody, GCKR antibody, Gckr antibody, gckr.L antibody

Background:

Description: Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.

Gene: GCKR

Molecular Weight: 68kDa

Gene ID: 2646

UniProt: Q14397

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus